Antibiotic producing microorganisms from River Wiwi, Lake Bosomtwe and the Gulf of Guinea at Doakor Sea Beach, Ghana

BMC Microbiology

Kofi Annan¹  Vivian E Boamah²  Francis Adu²  Stephen Y Gbedema²  Adelaide A Tawiah² 

[1] Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and technology, Kumasi, Ghana;Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and technology, Kumasi, Ghana

关键词: Multi-drug resistance;    Ghana;    Antibiotics;    Aquatic microorganisms;   

Others  :  1221708
DOI  :  10.1186/1471-2180-12-234

 received in 2012-02-20, accepted in 2012-09-27,  发布年份 2012

【 摘 要 】

Background

Microorganisms have provided a wealth of metabolites with interesting activities such as antimicrobial, antiviral and anticancer. In this study, a total of 119 aquatic microbial isolates from 30 samples (taken from water bodies in Ghana) were screened by the agar-well diffusion method for ability to produce antibacterial-metabolites.

Results

Antibacterial activity was exhibited by 27 of the isolates (14 bacteria, 9 actinomycetes and 4 fungi) against at least one of the indicator microorganisms: Enterococcus faecalis (ATCC 29212), Bacillus thuringiensis (ATCC 13838), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923), Proteus vulgaris (NCTC 4635) and Bacillus Subtilis (NCTC 10073). A sea isolate MAI2 (identified as a strain of Pseudomonas aeruginosa) exhibited the highest antibacterial activity (lowest zone of inhibition = 22 mm). The metabolites of MAI2 extracted with chloroform were stable to heat and gave minimum inhibitory concentrations ranging between 250 and 2000 μg/ml. Bioautography of the extract revealed seven active components.

Conclusion

This study has therefore uncovered the potential of water bodies in the West African sub-region as reservoirs of potent bioactive metabolite producing microorganisms.

【 授权许可】

   
2012 Tawiah et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Fenical W: Chemical studies of marine bacteria: developing a new resource. Chem Rev 1993, 93(5):1673-1683.
• [2]Singer RS, Finch R, Wegener HC, Bywater R, Walters J, Lipsitch M: Antibiotic resistance – the interplay between antibiotic use in animals and human beings. Lancet Infect Dis 2003, 3:47-51.
• [3]Bhavnani SM, Ballow CH: New agents for Gram-positive bacteria. Curr Op Microbiol 2000, 3:528-534.
• [4]Mincer TJ, Jensen PR, Kauffman CA, Fenical W: Widespread and persistent populations of a major new marine actinomycete taxon in ocean sediments. Appl Environ Microbiol 2002, 68(10):5005-5011.
• [5]Radajewski S, Webster G, Reay DS, Morris SA, Ineson P, Nedwell DB, Prosser JI, Murrell JC: Identification of active methylotroph populations in an acidic forest soil by stable isotope probing. Microbiol 2002, 148:2331-2342.
• [6]Prudhomme J, McDaniel E, Ponts N, Bertani S, Fenical W, Jensen P, Le Roch K: Marine Actinomycetes: a new source of compounds against the human malaria parasite. PLoS One 2008, 3(6):e2335.
• [7]Nostro A, Germanò M, D’Angelo V, Marino A, Cannatelli M: Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett Appl Microbiol 2000, 30:379-384.
• [8]Barrow GI, Felthan RKA: Cowan and Steel’s Manual for the Identification of Medical Bacteria. 3rd edition. Cambridge University Press, Cambridge UK; 2003:351-353.
• [9]Ivanova EP, Nicolau DV, Yumoto N, Taguchi T: Impact of conditions of cultivation and adsorption on antimicrobial activity of marine bacteria. Mar Biol 1998, 130:545-551.
• [10]Zheng L, Chen H, Han X, Lin W, Yan X: Antimicrobial screening and active compound isolation from marine bacterium NJ6-3-1 associated with the sponge Hymeniacidon perleve. World J Microbiol Biotechnol 2005, 21:201-206.
• [11]Brandelli A, Cladera-Olivera F, Motta SA: Screening for antimicrobial activity among bacteria isolated from the Amazon Basin. Braz J Microbiol 2004, 35:307-310.
• [12]O’Brien A, Sharp R, Russell NJ, Roller S: Antarctic bacteria inhibit growth of food-borne microorganisms at low temperatures. FEMS Microbiol Ecol 2004, 48(2):157-167.
• [13]Ampofo AJ: A survey of microbial pollution of rural domestic water supply in Ghana. Int J Environ Heal Res 1997, 7(2):121-130.
• [14]Boadi KO, Kuitumen M: Urban waste pollution in the Korle Lagoon, Accra, Ghana. Environmentalist 2002, 22(4):301-309.
• [15]Katte VY, Fonteh MF, Guemuh GN: Domestic water quality in urbancentres in Cameroon: a case study of Dschang in the West Province. African Water Journal 2003, 1:43-51.
• [16]Fianko JR, Osae S, Adomako D, Adotey DK, Serfo-Armah Y: Assessment of heavy metal pollution of the Iture Estuary in the Central region of Ghana. Environ Monit Assess 2007, 131(1–3):467-473.
• [17]Giudice AL, Bruni V, Michaud L: Characterization of Antarctic psychrotrophic bacteria with antibacterial activities against terrestrial microorganisms. J Basic Microbiol 2007, 47:496-505.
• [18]Bushell M, Grafe U: Bioactive metabolites from microorganisms. Industrial Microbiology 1989, 27:402-418.
• [19]Preetha RSJ, Prathapan S, Vijayan KK, Jayaprakash SN, Philip R, Singh BS: An inhibitory compound produced by Pseudomonas with effectiveness on Vibrio harveyi. Aquac Res 2009, 41:1452-1461.
• [20]Uzair B, Ahmed N, Kousar F, Edwards DH: Isolation and characterization of Pseudomonas strain that inhibit growth of indigenous and clinical isolates. The Internet Journal of Microbiology 2006, 2(2): . Available at: http://www.ispub.com/journal/the-internet-journal-of-microbiology webcite
• [21]Roitman J, Mahoney N, Janisiewicz W: Production and composition of phenylpyrrole metabolites produced by Pseudomonas cepacia. Appl Microbiol Biotechnol 1990, 34:381-386.
• [22]Price-Whelan A, Dietrich LEP, Newman DK: Rethinking secondary metabolism: Physiological roles for phenazine antibiotics. Nat Chem Biol 2006, 2:71-78.
• [23]Sole M, Francia A, Rius N, Loren JG: The role of pH in the glucose effet on prodigiosin production by non-proliferating cells of Serratia marcescens. Lett Applied Microbiol 1997, 25:81-84.
• [24]Merrick MJ, Edwards RA: Nitrogen control in bacteria. Microbiol Rev 1995, 59:604-622.
• [25]Shapiro S: Nitrogen assimilation in Actinomycetes and the influence of nitrogen nutrition on Actinomycetes secondary metabolism. In Regulation of Secondary Metabolism in Actinomycetes. Edited by Shapiro S. CRC Press, Boca Raton, Florida; 1989:135-211.
• [26]Charyulu ME, Gnanamani A: Condition stabilization for Pseudomonas aeruginosa MTCC 5210 to yield high Titres of extra cellular antimicrobial secondary metabolite using response surface methodology. Current Research in Bacteriology 2010, 4:197-213.
• [27]Garland PB: Energy transduction in microbial systems. Symp Soc Gen Microbiol 1977, 27:1-21.
• [28]Riebeling V, Thauer RK, Jungermann K: Internal-alkaline pH gradient, sensitive to uncoupler and ATPase inhibitor, in growing Clostridium pasteurianum. Eur J Biochem 1975, 55:445-453.
• [29]Chang SC, Wei YH, Wei DL, Chen YY, Jong SC: Factors affecting the production of eremofortin C and PR toxin in Penicillium roqueforti. Appl Environ Microbiol 1991, 57:2581-2585.
• [30]Gibbons S: Plants as a source of bacterial resistance modulators and anti-infective agents. Phytochem Rev 2005, 4:63-78.
• [31]Annan K, Adu F, Gbedema SY: Friedelin: a bacterial resistance modulator from Paullinia pinnata L. J Sci Technol 2009, 29(1):152-159.
• [32]Pankey GA, Sabath LD: Clinical relevance of bacteriostatic versus bactericidal mechanisms of action in the treatment of Gram-positive bacterial infections. Clin Infect Dis 2004, 38(6):864-870.
• [33]Van Lagevelde P, Van Dissel JT, Meurs CJC, Renz J, Groeneveld PHP: Combination of flucloxacillin and gentamicin inhibits toxic shock syndrome toxin 1 production by Staphylococcus aureus in both logarithmic and stationary phases of growth. Antimicrob Agents Chemother 1997, 41:1682-1685.
• [34]Russell NE, Pachorek RE: Clindamycin in the treatment of streptococcal and staphylococcal toxic shock syndromes. Ann Pharmacother 2000, 34(7–8):936-939.