MicroRNA-1246 enhances migration and invasion through CADM1 in hepatocellular carcinoma

BMC Cancer

Zhao Sun⁴  Changting Meng⁴  Shihua Wang¹  Na Zhou⁴  Mei Guan⁴  Chunmei Bai⁴  Shan Lu³  Qin Han¹  Robert Chunhua Zhao² 

[1] Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, People’s Republic of China

[2] Center of Translational medicine, Peking Union Medical College Hospital, Beijing, People’s Republic of China

[3] China National Center for Biotechnology Development, Beijing, People’s Republic of China

[4] Department of Oncology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China

关键词: CADM1;    MicroRNA-1246;    Invasion;    Hepatocellular carcinoma;   

Others  :  1134694
DOI  :  10.1186/1471-2407-14-616

 received in 2014-01-17, accepted in 2014-08-20,  发布年份 2014

【 摘 要 】

Background

The aberrant expression of microRNAs has been demonstrated to play a crucial role in the initiation and progression of hepatocarcinoma. miR-1246 expression in High invasive ability cell line than significantly higher than that in low invasive ability cell line.

Methods

Transwell chambers (8-uM pore size; Costar) were used in the in vitro migration and invison anssay. Dual luciferase reporter gene construct and Dual luciferase reporter assay to identify the target of miR-1246. CADM1 expression was evaluated by immunohistochemistric staining. The clinical manifestations, treatments and survival were collected for statistical analysis.

Results

Inhibition of miR-1246 effectively reduced migration and invasion of hepatocellular carcinoma cell lines. Bioinformatics and luciferase reporter assay revealed that miR-1246 specifically targeted the 3′-UTR of Cell adhesion molecule 1 and regulated its expression. Down-regulation of CADM1 enhanced migration and invasion of HCC cell lines. Furthermore, in tumor tissues obtained from liver cancer patients, the expression of miR-1246 was negatively correlated with CADM1 and the high expression of miR-1246 combined with low expression of CADM1 might serve as a risk factor for stage1 liver cancer patients.

Conclusions

Our study showed that miR-1246, by down-regulation CADM1, enhances migration and invasion in HCC cells.

【 授权许可】

   
2014 Sun et al.; licensee BioMed Central Ltd.

【 图 表 】

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