Towards microbiome transplant as a therapy for periodontitis: an exploratory study of periodontitis microbial signature contrasted by oral health, caries and edentulism

BMC Oral Health

Peter A. Noble⁴  Thomas F. Flemmig²  Thomas Beikler¹  Timothy W. Randolph³  Brian G. Leroux⁵  Alex E. Pozhitkov⁵ 

[1] Section of Periodontics, School of Medicine, Heinrich-Heine-University, Moorenstrasse 5, Düsseldorf, 40225, Germany;Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Sai Ying Pun, Hong Kong, SAR, Peoples’ Republic of China;Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Seattle 98109, WA, USA;PhD Program in Microbiology, Alabama State University, Montgomery 36101, AL, USA;Department of Oral Health Sciences, University of Washington, Seattle 98195-7444, WA, USA

关键词: Red complex;    Periodontitis;    Edentulism;    Caries;    Microbial transplant;    Bacteriotherapy;   

Others  :  1228336
DOI  :  10.1186/s12903-015-0109-4

 received in 2015-06-04, accepted in 2015-10-06,  发布年份 2015

【 摘 要 】

Background

Conventional periodontal therapy aims at controlling supra- and subgingival biofilms. Although periodontal therapy was shown to improve periodontal health, it does not completely arrest the disease. Almost all subjects compliant with periodontal maintenance continue to experience progressive clinical attachment loss and a fraction of them loses teeth. An oral microbial transplant may be a new alternative for treating periodontitis (inspired by fecal transplant). First, it must be established that microbiomes of oral health and periodontitis are distinct. In that case, the health-associated microbiome could be introduced into the oral cavity of periodontitis patients. This relates to the goals of our study: (i) to assess if microbial communities of the entire oral cavity of subjects with periodontitis were different from or oral health contrasted by microbiotas of caries and edentulism patients; (ii) to test in vitro if safe concentration of sodium hypochlorite could be used for initial eradication of the original oral microbiota followed by a safe neutralization of the hypochlorite prior transplantation.

Methods

Sixteen systemically healthy white adults with clinical signs of one of the following oral conditions were enrolled: periodontitis, established caries, edentulism, and oral health. Oral biofilm samples were collected from sub- and supra-gingival sites, and oral mucosae. DNA was extracted and 16S rRNA genes were amplified. Amplicons from the same patient were pooled, sequenced and quantified. Volunteer’s oral plaque was treated with saline, 16 mM NaOCl and NaOCl neutralized by ascorbate buffer followed by plating on blood agar.

Results

Ordination plots of rRNA gene abundances revealed distinct groupings for the oral microbiomes of subjects with periodontitis, edentulism, or oral health. The oral microbiome in subjects with periodontitis showed the greatest diversity harboring 29 bacterial species at significantly higher abundance compared to subjects with the other assessed conditions. Healthy subjects had significantly higher abundance in 10 microbial species compared to the other conditions. NaOCl showed strong antimicrobial properties; nontoxic ascorbate was capable of neutralizing the hypochlorite.

Conclusions

Distinct oral microbial signatures were found in subjects with periodontitis, edentulism, or oral health. This finding opens up a potential for a new therapy, whereby a health-related entire oral microbial community would be transplanted to the diseased patient.

【 授权许可】

   
2015 Pozhitkov et al.

【 图 表 】

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