Developments on drug discovery and on new therapeutics: highly diluted tinctures act as biological response modifiers

BMC Complementary and Alternative Medicine

Dorly de F Buchi¹  Edvaldo da S Trindade¹  Jenifer P Gonçalves¹  Eneida J Da Lozzo¹  Diogo Kuczera¹  Ediely L de O Coletto¹  Raffaello P Di Bernardi¹  Lucas F de Andrade¹  Fernando de SF Guimarães¹  Simone M de Oliveira¹  Ana Paula R Abud¹  Carolina C de Oliveira¹ 

[1] Laboratório de Estudos de Células Inflamatórias e Neoplásicas, Departamento de Biologia Celular, SCB, Centro Politecnico, Universidade Federal do Paraná - Curitiba, CEP 81531-980, PR - Brazil

关键词: macrophages;    lymph node cells;    bone marrow cells;    immune cells;    highly diluted tinctures;    drug discovery;   

Others  :  1232947
DOI  :  10.1186/1472-6882-11-101

 received in 2011-06-16, accepted in 2011-10-26,  发布年份 2011

【 摘 要 】

Background

In the search for new therapies novel drugs and medications are being discovered, developed and tested in laboratories. Highly diluted substances are intended to enhance immune system responses resulting in reduced frequency of various diseases, and often present no risk of serious side-effects due to its low toxicity. Over the past years our research group has been investigating the action of highly diluted substances and tinctures on cells from the immune system.

Methods

We have developed and tested several highly diluted tinctures and here we describe the biological activity of M1, M2, and M8 both in vitro in immune cells from mice and human, and in vivo in mice. Cytotoxicity, cytokines released and NF-κB activation were determined after in vitro treatment. Cell viability, oxidative response, lipid peroxidation, bone marrow and lymph node cells immunophenotyping were accessed after mice in vivo treatment.

Results

None of the highly diluted tinctures tested were cytotoxic to macrophages or K562. Lipopolysaccharide (LPS)-stimulated macrophages treated with all highly diluted tinctures decreased tumour necrosis factor alpha (TNF-α) release and M1, and M8 decreased IFN-γ production. M1 has decreased NF-κB activity on TNF-α stimulated reporter cell line. In vivo treatment lead to a decrease in reactive oxygen species (ROS), nitric oxide (NO) production was increased by M1, and M8, and lipid peroxidation was induced by M1, and M2. All compounds enhanced the innate immunity, but M1 also augmented acquired immunity and M2 diminished B lymphocytes, responsible to acquired immunity.

Conclusions

Based on the results presented here, these highly diluted tinctures were shown to modulate immune responses. Even though further investigation is needed there is an indication that these highly diluted tinctures could be used as therapeutic interventions in disorders where the immune system is compromised.

【 授权许可】

   
2011 de Oliveira et al; licensee BioMed Central Ltd.

【 图 表 】

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