The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation

BMC Developmental Biology

Ruijin Huang²  Beate Brand-Saberi³  Ketan Patel⁵  Michael Hans¹  Dieter Schwandulla¹  Carsten Theiss³  Maryna Masyuk³  Aisha Abduelmula³  Qin Pu⁴ 

[1] Institute of Physiology, Medical Faculty Bonn, Rheinish Friedrich-Wilhelms-University of Bonn, Bonn, Germany;Institute of Anatomy and Cell Biology, Department of Molecular Embryology, Medical Faculty, Albert- Ludwigs-University of Freiburg, Freiburg, Germany;Institute of Anatomy, Department of Anatomy and Molecular Embryology, Medical Faculty, Ruhr University of Bochum, Bochum, Germany;Institute of Anatomy, Department of Neuroanatomy, Medical Faculty Bonn, Rheinish Friedrich-Wilhelms-University of Bonn, Bonn, Germany;School Biological Sciences, University of Reading, Reading, UK

关键词: Chicken embryo;    Myotome;    Dermomyotome;    Somite;   

Others  :  1085432
DOI  :  10.1186/1471-213X-13-37

 received in 2013-03-22, accepted in 2013-10-11,  发布年份 2013

【 摘 要 】

Background

The myotome is the primitive skeletal muscle that forms within the embryonic metameric body wall. It can be subdivided into an epaxial and hypaxial domain. It has been shown that the formation of the epaxial myotome requires the dorsomedial lip of the dermomyotome (DML). Although the ventrolateral lip (VLL) of the dermomyotome is believed to be required for the formation of the hypaxial myotome, experimentally evidence for this statement still needs to be provided. Provision of such data would enable the resolution of a debate regarding the formation of the hypaxial dermomyotome. Two mechanisms have been proposed for this tissue. The first proposes that the intermediate dermomyotome undergoes cellular expansion thereby pushing the ventral lateral lip in a lateral direction (translocation). In contrast, the alternative view holds that the ventral lateral lip grows laterally.

Results

Using time lapse confocal microscopy, we observed that the GFP-labelled ventrolateral lip (VLL) of the dermomyotome grows rather than translocates in a lateral direction. The necessity of the VLL for lateral extension of the myotome was addressed by ablation studies. We found that the hypaxial myotome did not form after VLL ablation. In contrast, the removal of an intermediate portion of the dermomyotome had very little effect of the hypaxial myotome. These results demonstrate that the VLL is required for the formation of the hypaxial myotome.

Conclusion

Our study demonstrates that the dermomyotome ventrolateral lip is essential for the hypaxial myotome formation and supports the lip extension model. Therefore, despite being under independent signalling controls, both the dorsomedial and ventrolateral lip fulfil the same function, i.e. they extend into adjacent regions permitting the growth of the myotome.

【 授权许可】

   
2013 Pu et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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