Nature of bacterial colonization influences transcription of mucin genes in mice during the first week of life

BMC Research Notes

Tine R Licht²  Hanne Frøkiær¹  Lisbeth N Fink³  Stine B Metzdorff¹  Martin I Bahl²  Matilde B Kristensen²  Anders Bergström² 

[1] Department of Basic Sciences and Environment, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark;Gut Ecology Group, Department of Food Microbiology, National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, Søborg, 2860, Denmark;Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark

关键词: 16S rRNA;    Escherichia coli Nissle;    Lactobacillus acidophilus NCFM;    Probiotics;    Postnatal transcription onset;    LinRegPCR;    qPCR;    Monocolonized;    Germ free mice;   

Others  :  1166017
DOI  :  10.1186/1756-0500-5-402

 received in 2011-11-16, accepted in 2012-07-12,  发布年份 2012

【 摘 要 】

Background

Postnatal regulation of the small intestinal mucus layer is potentially important in the development of adult gut functionality. We hypothesized that the nature of bacterial colonization affects mucus gene regulation in early life.

We thus analyzed the influence of the presence of a conventional microbiota as well as two selected monocolonizing bacterial strains on the transcription of murine genes involved in mucus layer development during the first week of life.

Mouse pups (N = 8/group) from differently colonized dams: Germ-free (GF), conventional specific pathogen free (SPF), monocolonized with either Lactobacillus acidophilus NCFM (Lb) or Escherichia coli Nissle (Ec) were analyzed by qPCR on isolated ileal tissue sections from postnatal days 1 and 6 (PND1, PND6) after birth with respect to: (i) transcription of specific genes involved in mucus production (Muc1-4, Tff3) and (ii) amounts of 16S rRNA of Lactobacillus and E. coli. Quantification of 16S rRNA genes was performed to obtain a measure for amounts of colonized bacteria.

Results

We found a microbiota-independent transcriptional increase of all five mucus genes from PND1 to PND6. Furthermore, the relative level of transcription of certain mucus genes on PND1 was increased by the presence of bacteria. This was observed for Tff3 in the SPF, Ec, and Lb groups; for Muc2 in SPF; and for Muc3 and Muc4 in Ec and Lb, respectively.

Detection of bacterial 16S rRNA genes levels above the qPCR detection level occurred only on PND6 and only for some of the colonized animals. On PND6, we found significantly lower levels of Muc1, Muc2 and Muc4 gene transcription for Lb animals with detectable Lactobacillus levels as compared to animals with Lactobacillus levels below the detection limit.

Conclusions

In summary, our data show that development of the expression of genes encoding secreted (Muc2/Tff3) and membrane-bound (Muc1/Muc3/Muc4) mucus regulatory proteins, respectively, is distinct and that the onset of this development may be accelerated by specific groups of bacteria present or absent at the mucosal site.

【 授权许可】

   
2012 Bergström et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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