Investigation of the mechanisms of Angelica dahurica root extract-induced vasorelaxation in isolated rat aortic rings

BMC Complementary and Alternative Medicine

Ho-Young Choi¹  Inhye Ham¹  Min Sik Shin¹  Kyungjin Lee¹ 

[1] Department of Herbology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Republic of Korea

关键词: hypertension;    voltage-dependent calcium channel;    receptor-operated calcium channel;    vasorelaxant effect;    Angelica dahurica;   

Others  :  1233207
DOI  :  10.1186/s12906-015-0889-8

 received in 2015-05-06, accepted in 2015-10-02,  发布年份 2015

【 摘 要 】

Background

The root of Angelica dahurica Bentham et Hooker (Umbelliferae) has been used as a traditional medicine for colds, headache, dizziness, toothache, supraorbital pain, nasal congestion, acne, ulcer, carbuncle, and rheumatism in China, Japan, and Korea. Interestingly, it has been used in the treatment of vascular diseases including hypertension. The aim of this study was to provide pharmacological evidence for the anti-hypertensive effect of A. dahurica by investigating the mechanism underlying its vasorelaxant effect.

Methods

The vasorelaxant effects of a 70 % methanol extract of the A. dahurica root (ADE) on rat thoracic aorta and its underlying mechanisms were assessed. Isolated rat aortic rings were suspended in organ chambers containing 10 ml Krebs-Henseleit (K-H) solution and placed between 2 tungsten stirrups and connected to an isometric force transducer. Changes in tension were recorded via isometric transducers connected to a data acquisition system.

Results

ADE causes concentration-dependent relaxation in both endothelium-intact and endothelium-denuded aortic rings precontracted with phenylephrine (PE; 1 μM) or potassium (KCl; 60 mM) in K-H solution. And pre-treatment with ADE (1 mg/ml) inhibited calcium-induced vasocontraction of aortic rings induced by PE or KCl. However, ADE pre-treatment did not affect the contraction induced by PE or caffeine in Ca ²⁺ -free K-H solution.

Conclusions

These results suggested that the ADE has vasorelaxant effect and the vasorelaxant activity is mediated by endothelium-independent pathway that includes the blockade of extracellular calcium influx through the receptor-operated Ca ²⁺channel and voltage-dependent calcium channel pathways.

【 授权许可】

   
2015 Lee et al.

【 图 表 】

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