Activation of defence pathways in Scots pine bark after feeding by pine weevil (Hylobius abietis)

BMC Genomics

Fred O Asiegbu¹  Jarmo K Holopainen³  Jeffrey FD Dean²  W Walter Lorenz²  Rajendra Ghimire³  Susanna Keriö¹  Emad Jaber¹  Tommaso Raffaello¹  Andriy Kovalchuk¹ 

[1] Department of Forest Sciences, University of Helsinki, Helsinki, FIN-00014, Finland;Warnell School of Forestry and Natural Resources, The University of Georgia, Athens 30602, GA, USA;Department of Environmental Science, University of Eastern Finland, Kuopio, FIN-70211, Finland

关键词: PR proteins;    Protease inhibitors;    Terpenoid pathway;    Phenylpropanoid pathway;    Transcriptomics;    VOC emission;    Herbivory;   

Others  :  1204051
DOI  :  10.1186/s12864-015-1546-9

 received in 2014-09-04, accepted in 2015-04-17,  发布年份 2015

【 摘 要 】

Background

During their lifetime, conifer trees are exposed to numerous herbivorous insects. To protect themselves against pests, trees have developed a broad repertoire of protective mechanisms. Many of the plant’s defence reactions are activated upon an insect attack, and the underlying regulatory mechanisms are not entirely understood yet, in particular in conifer trees. Here, we present the results of our studies on the transcriptional response and the volatile compounds production of Scots pine (Pinus sylvestris) upon the large pine weevil (Hylobius abietis) feeding.

Results

Transcriptional response of Scots pine to the weevil attack was investigated using a novel customised 36.4 K Pinus taeda microarray. The weevil feeding caused large-scale changes in the pine transcriptome. In total, 774 genes were significantly up-regulated more than 4-fold (p ≤ 0.05), whereas 64 genes were significantly down-regulated more than 4-fold. Among the up-regulated genes, we could identify genes involved in signal perception, signalling pathways, transcriptional regulation, plant hormone homeostasis, secondary metabolism and defence responses. The weevil feeding on stem bark of pine significantly increased the total emission of volatile organic compounds from the undamaged stem bark area. The emission levels of monoterpenes and sesquiterpenes were also increased. Interestingly, we could not observe any correlation between the increased production of the terpenoid compounds and expression levels of the terpene synthase-encoding genes.

Conclusions

The obtained data provide an important insight into the transcriptional response of conifer trees to insect herbivory and illustrate the massive changes in the host transcriptome upon insect attacks. Moreover, many of the induced pathways are common between conifers and angiosperms. The presented results are the first ones obtained by the use of a microarray platform with an extended coverage of pine transcriptome (36.4 K cDNA elements). The platform will further facilitate the identification of resistance markers with the direct relevance for conifer tree breeding.

【 授权许可】

   
2015 Kovalchuk et al.; licensee BioMed Central.

【 图 表 】

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