Characterizing the neurotranscriptomic states in alternative stress coping styles

BMC Genomics

John Godwin²  Melissa S. Lamm²  Ryan Y. Wong¹ 

[1] Current Address: Department of Biology, University of Nebraska at Omaha, Omaha NE 68182, USA;Department of Biological Sciences, W.M. Keck Center for Behavioral Biology, North Carolina State University, Raleigh NC 27695-7614, USA

关键词: Gene coexpression network;    RNA-sequencing;    Transcriptome;    Danio rerio;    Brain;    Anxiety;    Reactive;    Proactive;    Coping style;    Stress;   

Others  :  1210195
DOI  :  10.1186/s12864-015-1626-x

 received in 2015-02-16, accepted in 2015-05-08,  发布年份 2015

【 摘 要 】

Background

Animals experience stress in many contexts and often successfully cope. Individuals exhibiting the proactive versus reactive stress coping styles display qualitatively different behavioral and neuroendocrine responses to stressors. The predisposition to exhibiting a particular coping style is due to genetic and environmental factors. In this study we explore the neurotranscriptomic and gene network biases that are associated with differences between zebrafish (Danio rerio) lines selected for proactive and reactive coping styles and reared in a common garden environment.

Results

Using RNA-sequencing we quantified the basal transcriptomes from the brains of wild-derived zebrafish lines selectively bred to exhibit the proactive or reactive stress coping style. We identified 1953 genes that differed in baseline gene expression levels. Weighted gene coexpression network analyses identified one gene module associated with line differences. Together with our previous pharmacological experiment, we identified a core set of 62 genes associated with line differences. Gene ontology analyses reveal that many of these core genes are implicated in neurometabolism (e.g. organic acid biosynthetic and fatty acid metabolic processes).

Conclusions

Our results show that proactive and reactive stress coping individuals display distinct basal neurotranscriptomic states. Differences in baseline expression of select genes or regulation of specific gene modules are linked to the magnitude of the behavioral response and the display of a coping style, respectively. Our results expand the molecular mechanisms of stress coping from one focused on the neurotransmitter systems to a more complex system that involves an organism’s capability to handle neurometabolic loads and allows for comparisons with other animal taxa to uncover potential conserved mechanisms.

【 授权许可】

   
2015 Wong et al.; licensee BioMed Central.

【 图 表 】

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