Clinically helpful rickettsial disease diagnostic IgG titers in relation to duration of illness in an endemic setting in Sri Lanka

BMC Research Notes

H Janaka de Silva¹  Gregory A Dasch³  Narasinghe W Bandara²  TGA Nilmini Chandrasena⁴  Amanda Ranaweera¹  Sanjaya Weerasinghe¹  Ranjan Premaratna¹ 

[1] Department of Medicine, Faculty of Medicine, University of Kelaniya, Colombo, Sri Lanka;Department of Microbiology, Faculty of Medicine, University of Kelaniya, Colombo, Sri Lanka;Rickettsial Zoonoses Branch, CDC, Atlanta, GA, USA;Department of Parasitology, Faculty of Medicine, University of Kelaniya, Colombo, Sri Lanka

关键词: Spotted fever;    Orientia tsutsugamushi;    Sri Lanka;    Endemic;    IFA-IgG;    Diagnosis Rickettsioses;   

Others  :  1165093
DOI  :  10.1186/1756-0500-5-662

 received in 2011-09-22, accepted in 2012-10-23,  发布年份 2012

【 摘 要 】

Background

Although an initial IFA-IgG titer greater or equal to 1/64 or 1/128 is considered positive in presumptive diagnosis, in clinical practice in an endemic setting for rickettsioses in Sri Lanka, some patients with IFA-IgG titer of 1/128 for either spotted fever group (SFG) or scrub typhus (ST) did not respond to treatment.

Findings

To determine a clinically helpful diagnostic algorithm, IFA-IgG results of serologically confirmed treatment responders were analyzed in relation to duration of illness at sampling. Of 146 suspected SFG, 3 responders of 25 patients had titers ≤1/128 with < 7 days of illness while all 9 with titers ≥1/256 responded (false negative with 1/256 cutoff was 12%, false positive was 0%). For illness > 7 days, the false negative and positive rates were 4.3% (3/59) and 11.3% (6/53). Of 115 suspected ST, false negative and positive rates with ≥1/256 cutoff at <7 days of illness were 14.2% (2/14) and 0% (0/8) respectively while > 7 days, false negative and positive rates were 2% (1/51) and 0% (0/42).

Conclusions

For clinical decision making, duration of illness at sampling is important in interpreting serology results in an endemic setting. If sample is obtained ≤7 day of illness, an IgG titer of ≤1/128 requires a follow up sample in the diagnosis and > 7 days of illness, a single ≥1/256 titer is diagnostic for all ST and 90% of SFG.

【 授权许可】

   
2012 Premaratna et al.; licensee BioMed Central Ltd.

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