Paclobutrazol treatment as a potential strategy for higher seed and oil yield in field-grown camelina sativa L. Crantz

BMC Research Notes

Attipalli R Reddy¹  BSK Chaitanya¹  Anirban Guha¹  Jella Satyanarayana²  Sreenivas Ghatty³  Sumit Kumar¹ 

[1] Department of Plant Sciences, University of Hyderabad, Hyderabad 500046, India;Department of Environment, A.N.G.R. Agricultural University, Hyderabad 500030, India;Tree Oils India Limited (TOIL), Zaheerabad 502226, India

关键词: Biofuel Camelina sativa Growth Paclobutrazol Physiology Seed yield;   

Others  :  1166600
DOI  :  10.1186/1756-0500-5-137

 received in 2011-09-29, accepted in 2012-03-13,  发布年份 2012

【 摘 要 】

Background

Camelina (Camelina sativa L. Crantz) is a non-food oilseed crop which holds promise as an alternative biofuel energy resource. Its ability to grow in a variety of climatic and soil conditions and minimal requirements of agronomical inputs than other oilseed crops makes it economically viable for advanced biofuel production. We designed a study to investigate the effect of paclobutrazol [2RS, 3RS)-1-(4-Chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pentan-3-ol] (PBZ), a popular plant growth regulator, on the seed and oil yield of Camelina sativa (cv. Celine).

Results

A field-based micro-trial setup was established in a randomized block design and the study was performed twice within a span of five months (October 2010 to February 2011) and five different PBZ treatments (Control: T₀; 25 mg l^-1: T₁; 50 mg l^-1: T₂; 75 mg l^-1: T₃; 100 mg l^-1: T₄; 125 mg l^-1: T₅) were applied (soil application) at the time of initiation of flowering. PBZ at 100 mg l^-1 concentration (T₄) resulted in highest seed and oil yield by 80% and 15%, respectively. The seed yield increment was mainly due to enhanced number of siliques per plant when compared to control. The PBZ - treated plants displayed better photosynthetic leaf gas exchange characteristics, higher chlorophyll contents and possessed dark green leaves which were photosynthetically active for a longer period and facilitated higher photoassimilation.

Conclusion

We report for the first time that application of optimized PBZ dose can be a potential strategy to achieve higher seed and oil yield from Camelina sativa that holds great promise as a biofuel crop in future.

【 授权许可】

   
2012 Reddy et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

【 参考文献 】

• [1]Moser BR: Camelina (Camelina sativ L.) oils as a biofuels feedstock: Golden opportunity or false hope? Lipid Tech 2010, 22:270-273.
• [2]Shonnard DR, Williams L, Kalnes TN: Camelina-Derived Jet Fuel and Diesel: Sustainable Advanced Biofuels. Environ Prog Sustain Energy 2010, 29:382-392.
• [3]Zubr J: Qualitative variation of Camelina sativ seed from different locations. Ind Crop Prod 2003, 17:161-169.
• [4]Pan BZ, Xu ZF: Benzyladenine Treatment Significantly Increases the Seed Yield of the Biofuel Plant Jatropha curca. J Plant Growth Regul 2011, 30:166-174.
• [5]Abdelgadir HA, Johnson SD, Staden JV: Promoting branching of a potential biofuel crop Jatropha curca L. by foliar application of plant growth regulators. Plant Growth Regul 2009, 58:287-295.
• [6]Davis TD, Steffens GL, Sankhla N: Triazol plant growth regulators. Hortic Rev 1988, 10:151-188.
• [7]Asin L, Alegre S, Montserrat R: Effect of paclobutrazol, prohexadione-Ca, deficit irrigation, summer pruning and root pruning on shoot growth, yield, and return bloom, in a 'Blanquilla' pear orchard. Sci Hortic 2007, 113:142-148.
• [8]Berova M, Zlatev Z: Physiological response and yield of paclobutrazol treated tomato plants (Lycopersicon esculentu Mill.). Plant Growth Regul 2000, 30:117-123.
• [9]Blaikie SJ, Kulkarni VJ, Müller WJ: Effects of morphactin and paclobutrazol flowering treatments on shoot and root phenology in mango cv. Kensington Pride. Sci Hortic 2004, 101:51-68.
• [10]Ghosh A, Chikara J, Chaudhary DR, Prakash AR, Boricha G, Zala A: Paclobutrazol Arrests Vegetative Growth and Unveils Unexpressed Yield Potential of Jatropha curca. J Plant Growth Regul 2010, 29:307-315.
• [11]Senoo S, Isoda A: Effects of Paclobutrazol on Dry matter Distribution and Yield in Peanut. Plant Prod Sci 2003, 6:90-94.
• [12]Setia RC, Bhathal G, Setia N: Influence of paclobutrazol on growth and yield of Brassica carinat A.Br. Plant Growth Regul 1995, 16:121-127.
• [13]Senoo S, Isoda A, Nojima H, Takasaki Y: Effects of Paclobutrazol on flowering and seed-setting habit and physiological, morphological characters in peanut. Jpn J Crop Sci 2001, 70:188-189.
• [14]Gao J, Hofstra G, Fletcher RA: Anatomical changes induced by triazoles in wheat seedlings. Can J Bot 1987, 66:1178-1185.
• [15]Christov C, Tsvetkov I, Kovachev V: Use of paclobutrazol to control vegetative growth and improve fruiting efficiency of grapevines (Vitis vinifera L.). Bulg J Plant Physiol 1995, 21:64-71.
• [16]Kende H: The cytokinins. Int Rev Cytol 1971, 31:301-338.
• [17]Farquhar GD, Sharkey TD: Stomatal conductance and photosynthesis. Annu Rev Plant Physiol Plant Mol Biol 1982, 33:317-345.
• [18]Dalziel J, Lawrence DK: Biochemical and biological effects of kaurene oxidase inhibitors, such as paclobutrazol. In British Plant Growth Regulator Group. Monograph 1984, 11:43-57.
• [19]Belakbir A: Yield and fruit quality of pepper (Capsicum annu L.) in response to bioregulators. HortScience 1998, 33:85-87.
• [20]Monge E, Aguirre R, Blanco A: Application of Paclobutrazol and GA3 to Adult Peach Trees: Effects on Nutritional Status and Photosynthetic Pigments. J Plant Growth Regul 1994, 13:15-19.
• [21]Sebastian B, Alberto G, Emilio AC, Jose AF, Juan AF: Growth development and color response of potted Dianthus caryophyllu to paclobutrazol treatment. Sci Hortic 2002, 1767:1-7.
• [22]Chaney WR: Tree Growth Retardants: Arborists discovering new uses for an old tool. Tree Care Industry Magazine 2003, 54:2-6.
• [23]Bruinsma J: Plant growth regulation in field crops. In Chemical Manipulation of crop growth and development. Edited by McLaren JS. London, Butterworth Scientific; 1982:3-11.
• [24]Grossmann K: Plant growth retardants as tools in physiological research. Physiol Plant 1990, 78:640-648.
• [25]Noodén LD, Leopold AC: Senescence and Aging in Plants. San Diego: Academic; 1988.
• [26]Rademacher W: Inhibitors of gibberellin biosynthesis: applications in agriculture and horticulture. In Gibberellins. Edited by Takahashi N, Phinney BO, MacMillan J. New York, Springer-Verlag Inc.; 1990:296-310.
• [27]Synková H, Semorádová Š, Schnablová R, Witters E, Hušák M, Valcke R: Cytokinin-induced activity of antioxidant enzymes in transgenic Pssu-ip tobacco during plant ontogeny. Biol Plantarum 2006, 50:31-41.
• [28]Campbell NA, Reece JB, Urry LA, Cain ML, Wasserman SA, Minorsky PV, Jackson RB: Biology. eighth edition. Benjamin Cummings, San Francisco: Pearson; 2008:827-830.
• [29]Deneke CF, Keever GJ: Comparison of application methods of paclobutrazol for height control of potted tulips. HortScience 1992, 27:1329.
• [30]Bañón S, González A, Cano EA, Franco JA, Fernández JA: Growth, development and colour response of potted Dianthus caryophyllu cv. Mondriaan to paclobutrazol treatment. Sci Hortic 2002, 94:371-377.
• [31]Yeshitela T, Robbertse PJ, Stassen PJC: Paclobutrazol suppressed vegetative growth and improved yield as well as fruit quality of 'Tommy Atkins' mango (Mangifera indic) in Ethiopia. N Z J Crop Hortic Sci 2004, 32:281-293.
• [32]Woodward EJ, Marshall C: Effect of plant growth regulators and nutrient supply on tiller bud outgrowth in barley (Hordeum distichu L.). Ann Bot 1988, 61:347-354.
• [33]Khryanin VN: Evolution of the Pathways of Sex Differentiation in Plants. Russ J Plant Physiol 2007, 54:845-852.
• [34]Kurian RM, Iyer CPA: Stem anatomical characteristics in relation to tree vigour in mango (Mangifera indic L.). Sci Hortic 1992, 50:245-253.
• [35]Voon CH, Pitakpaivan C, Tan SJ: Mango cropping manipulation with Cultar. Acta Hortic 1991, 291:219-228.
• [36]Addo-Quaye AA, Daniels RW, Scarisbrick DH: The influence of paclobutrazol on the distribution and utilization of 14C-labelled assimilate fixed at anthesis in oilseed rape (Brassica napu L.). J Agric Sci (Cambridge) 1985, 105:365-373.
• [37]Hiscox JD, Isrealstam GF: A method for extraction of chlorophyll from leaf tissue without maceration. Can J Bot 1979, 57:1332-1334.
• [38]Arnon DI: Copper enzymes in isolated Chloroplast. Polyphenol oxidase in Beta vulgari. Plant Physiol 1949, 24:1-15.
• [39]AOAC (Association of Official Analytical Chemists): Official methods of analysis of AOAC International. sixteenth edition. Arlington, VA, USA; 1995.
• [40]Lui H, Lui YZ, Yang MF, Shen SH: A comparative analysis of embryo and endosperm proteome from seeds of Jatropha curca. J Integr Plant Biol 2009, 51:850-857.
• [41]Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Ann Biochem 1976, 72:248-253.