MicroRNA expression profiling of the fifth-instar posterior silk gland of Bombyx mori

BMC Genomics

Boxiong Zhong³  Jun Yu²  Zhengying You³  Jiaqian Che³  Shaohua Wang³  Lupeng Ye³  Yimei Cai²  Jisheng Li¹ 

[1]Institute of Sericulture, Chengde Medical University, Chengde 067000, P.R. China

[2]Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100029, China

[3]College of Animal Sciences, Zhejiang University, Hangzhou, Hangzhou 310058, P.R. China

关键词: Target gene;    Posterior silk gland;    Silkworm;    MicroRNA;   

Others  :  1217186
DOI  :  10.1186/1471-2164-15-410

 received in 2013-12-19, accepted in 2014-05-07,  发布年份 2014

【 摘 要 】

Background

The growth and development of the posterior silk gland and the biosynthesis of the silk core protein at the fifth larval instar stage of Bombyx mori are of paramount importance for silk production.

Results

Here, aided by next-generation sequencing and microarry assay, we profile 1,229 microRNAs (miRNAs), including 728 novel miRNAs and 110 miRNA/miRNA* duplexes, of the posterior silk gland at the fifth larval instar. Target gene prediction yields 14,222 unique target genes from 1,195 miRNAs. Functional categorization classifies the targets into complex pathways that include both cellular and metabolic processes, especially protein synthesis and processing.

Conclusion

The enrichment of target genes in the ribosome-related pathway indicates that miRNAs may directly regulate translation. Our findings pave a way for further functional elucidation of these miRNAs and their targets in silk production.

【 授权许可】

   
2014 Li et al.; licensee BioMed Central Ltd.

【 图 表 】

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