Intermittent whole-body vibration attenuates a reduction in the number of the capillaries in unloaded rat skeletal muscle

BMC Musculoskeletal Disorders

Kaoru Yamaoka²  Tomoyuki Kurose¹  Seiichi Kawamata¹  Junya Ozawa²  Akinori Kaneguchi³ 

[1] Graduate School of Biomedical & Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-Ku, Hiroshima, Hiroshima, Japan;Department of Rehabilitation, Faculty of Rehabilitation, Hiroshima International University, 555-36, Gakuendai, Kurose, Higashi-Hiroshima, Hiroshima, Japan;Major in Medical Engineering and Technology, Graduate School of Medical Technology and Health Welfare Science, Hiroshima International University, 555-36, Gakuendai, Kurose, Higashi-Hiroshima, Hiroshima, Japan

关键词: Weight bearing;    Whole-body vibration;    Muscle atrophy;    Angiogenic factor;    Capillary;    Skeletal muscle;    Hindlimb suspension;   

Others  :  1122067
DOI  :  10.1186/1471-2474-15-315

 received in 2014-04-04, accepted in 2014-09-22,  发布年份 2014

【 摘 要 】

Background

Whole-body vibration has been suggested for the prevention of muscle mass loss and muscle wasting as an attractive measure for disuse atrophy. This study examined the effects of daily intermittent whole-body vibration and weight bearing during hindlimb suspension on capillary number and muscle atrophy in rat skeletal muscles.

Methods

Sixty male Wistar rats were randomly divided into four groups: control (CONT), hindlimb suspension (HS), HS + weight bearing (WB), and HS + whole-body vibration (VIB) (n = 15 each). Hindlimb suspension was applied for 2 weeks in HS, HS + WB, and HS + VIB groups. During suspension, rats in HS + VIB group were placed daily on a vibrating whole-body vibration platform for 20 min. In HS + WB group, suspension was interrupted for 20 min/day, allowing weight bearing. Untreated rats were used as controls.

Results

Soleus muscle wet weights and muscle fiber cross-sectional areas (CSA) significantly decreased in HS, HS + WB, and HS + VIB groups compared with CONT group. Both muscle weights and CSA were significantly greater in HS + WB and HS + VIB groups compared with HS group. Capillary numbers (represented by capillary-to-muscle fiber ratio) were significantly smaller in all hindlimb suspension-treated groups compared with CONT group. However, a reduction in capillary number by unloading hindlimbs was partially prevented by whole-body vibration. These findings were supported by examining mRNA for angiogenic-related factors. Expression levels of a pro-angiogenic factor, vascular endothelial growth factor-A mRNA, were significantly lower in all hindlimb suspension-treated groups compared with CONT group. There were no differences among hindlimb suspension-treated groups. Expression levels of an anti-angiogenic factor, CD36 (receptor for thrombospondin-1) mRNA, were significantly higher in all hindlimb suspension-treated groups compared with CONT group. Among the hindlimb suspension-treated groups, expression of CD36 mRNA in HS + VIB group tended to be suppressed (less than half the HS group).

Conclusions

Our results suggest that weight bearing with or without vibration is effective for disuse-derived disturbance by preventing muscle atrophy, and whole-body vibration exercise has an additional benefit of maintaining microcirculation of skeletal muscle.

【 授权许可】

   
2014 Kaneguchi et al.; licensee BioMed Central Ltd.

【 图 表 】

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