Ubiquinol decreases monocytic expression and DNA methylation of the pro-inflammatory chemokine ligand 2 gene in humans

BMC Research Notes

Frank Döring²  Constance Schmelzer¹  Simone Onur²  Alexandra Fischer² 

[1] Research Unit Nutritional Physiology “Oskar Kellner”, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany;Institute for Human Nutrition and Food Science, Department of Molecular Prevention, Christian Albrechts University, Kiel, Germany

关键词: Inflammation;    DNA methylation;    Gene expression;    Ubiquinol;    Coenzyme Q10;   

Others  :  1165555
DOI  :  10.1186/1756-0500-5-540

 received in 2012-04-03, accepted in 2012-09-20,  发布年份 2012

【 摘 要 】

Background

Coenzyme Q₁₀ is an essential cofactor in the respiratory chain and serves in its reduced form, ubiquinol, as a potent antioxidant. Studies in vitro and in vivo provide evidence that ubiquinol reduces inflammatory processes via gene expression. Here we investigate the putative link between expression and DNA methylation of ubiquinol sensitive genes in monocytes obtained from human volunteers supplemented with 150 mg/ day ubiquinol for 14 days.

Findings

Ubiquinol decreases the expression of the pro-inflammatory chemokine (C-X-C motif) ligand 2 gene (CXCL2) more than 10-fold. Bisulfite-/ MALDI-TOF-based analysis of regulatory regions of the CXCL2 gene identified six adjacent CpG islands which showed a 3.4-fold decrease of methylation status after ubiquinol supplementation. This effect seems to be rather gene specific, because ubiquinol reduced the expression of two other pro-inflammatory genes (PMAIP1, MMD) without changing the methylation pattern of the respective gene.

Conclusion

In conclusion, ubiquinol decreases monocytic expression and DNA methylation of the pro-inflammatory CXCL2 gene in humans. Current Controlled Trials ISRCTN26780329.

【 授权许可】

   
2012 Fischer et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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