Molecular epidemiology and characterization of an outbreak causing Klebsiella pneumoniae clone carrying chromosomally located bla CTX-M-15 at a German University-Hospital

BMC Microbiology

Can Imirzalioglu²  Trinad Chakraborty²  Torsten Hain²  Eugen Domann²  Linda Falgenhauer²  Moritz Fritzenwanker²  Stephen E. Mshana¹ 

[1] Catholic University of Health and Allied Sciences, Weill Bugando School of Medicine Box, Mwanza, 1464, Tanzania;German Center for Infection Research (DZIF), Partner site Giessen-Marburg-Langen, Campus Giessen, Schubertstrasse 81, Giessen, 35392, Germany

关键词: Chromosomal insertion;    Klebsiella;    Multi-resistance;    ESBL;    Nosocomial infection;    Hospital;   

Others  :  1221664
DOI  :  10.1186/s12866-015-0460-2

 received in 2015-01-15, accepted in 2015-06-01,  发布年份 2015

【 摘 要 】

Background

Multi-drug resistant Klebsiella pneumoniae strains are a common cause of health care associated infections worldwide. Clonal spread of Klebsiella pneumoniae isolates carrying plasmid mediated CTX-M-15 have been commonly reported. Limited data is available regarding dissemination of chromosomally encoded CTX-M-15 in Klebsiella pneumoniae worldwide.

Results

We examined 23 non-repetitive ESBL-producing Klebsiella pneumoniae strains isolated from clinical specimens over a period of 4 months in a German University Hospital. All isolates were characterized to determine their genetic relatedness using Pulsed-Field Gel Electrophoresis (PFGE) and Multi Locus Sequence Typing (MLST). PFGE revealed three clusters (B1, B2, and B3) with a sub-cluster (A3) comprising of 10 isolates with an identical PFGE pattern. All strains of the cluster B3 with similar PFGE patterns were typed as ST101, indicating an outbreak situation. The ESBL allele bla_CTX-M-15 was identified in 16 (69.6 %) of all isolates, including all of the outbreak strains. Within the A3 sub-cluster, the CTX-M-15 allele could not be transferred by conjugation. DNA hybridization studies suggested a chromosomal location of bla_CTX-M-15. Whole genome sequencing located CTX-M-15 within a complete ISEcp-1 transposition unit inserted into an ORF encoding for a putative membrane protein. PCR-based analysis of the flanking regions demonstrated that insertion into this region is unique and present in all outbreak isolates.

Conclusion

This is the first characterization of a chromosomal insertion of bla_CTX-M-15 in Klebsiella pneumonia ST101, a finding suggesting that in Enterobacteriaceae, chromosomal locations may also act as reservoirs for the spread of bla_CTX-M-15 encoding transposition units.

【 授权许可】

   
2015 Mshana et al.

【 图 表 】

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