Effect of epimedium pubescen flavonoid on bone mineral status and bone turnover in male rats chronically exposed to cigarette smoke

BMC Musculoskeletal Disorders

Guang-hua Lei²  Qiang Xie⁴  Jin-peng Sun⁴  Chao Zeng⁴  Jian Tian⁴  Yi-lin Xiong⁴  Wen-feng Xiao⁴  Fang-jie Zhang⁴  Li-Cheng Wei⁴  Wei Jiang⁴  Mai Xu⁴  Wen-He Liu¹  Kang-hua Li²  Ling Cheng³  Shu-guang Gao⁴ 

[1] Department of Orthopaedics, Affiliated Hospital of Xiangnan University, Chenzhou, Hunan, China;Orthopaedics Institute of Central South University, Changsha, Hunan, China;Department of Medical Record and Information, Xiangya Hospital, Central South University, No.87 Xiangya Road, Changsha, Hunan, 410008, China;Department of Orthopaedics, Xiangya Hospital, Central South University, No.87 Xiangya Road, Changsha 410008, Hunan, China

关键词: Bone histomorphometry;    Bone turnover;    Bone mineral content;    Bone mineral density;    Epimedium pubescen flavonoid;    Smoking;   

Others  :  1149414
DOI  :  10.1186/1471-2474-13-105

 received in 2012-01-19, accepted in 2012-05-28,  发布年份 2012

【 摘 要 】

Background

Epimedii herba is one of the most frequently used herbs in formulas that are prescribed for the treatment of osteoporosis in China and its main constituent is Epimedium pubescen flavonoid (EPF). However, it is unclear whether EPF during chronic exposure to cigarette smoke may have a protective influence on the skeleton. The present study investigated the effect of EPF on bone mineral status and bone turnover in a rat model of human relatively high exposure to cigarette smoke.

Methods

Fifty male Wistar rats were randomized into five groups: controls, passive smoking groups and passive smoking rats administered EPF at three dosage levels (75, 150 or 300 mg/kg/day) in drinking water for 4 months. A rat model of passive smoking was prepared by breeding male rats in a cigarette-smoking box. Bone mineral content (BMC), bone mineral density (BMD), bone turnover markers, bone histomorphometric parameters and biomechanical properties were examined.

Results

Smoke exposure decreased BMC and BMD, increased bone turnover (inhibited bone formation and stimulated its resorption), affected bone histomorphometry (increased trabecular separation and osteoclast surface per bone surface; decreased trabecular bone volume, trabecular thickness, trabecular number, cortical thickness, bone formation rate and osteoblast surface per bone surface), and reduced mechanical properties. EPF supplementation during cigarette smoke exposure prevented smoke-induced changes in bone mineral status and bone turnover.

Conclusion

The results suggest that EPF can prevent the adverse effects of smoke exposure on bone by stimulating bone formation and inhibiting bone turnover and bone resorption.

【 授权许可】

   
2012 Gao et al.; licensee BioMed Central Ltd.

【 图 表 】

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