Short-term responses of unicellular planktonic eukaryotes to increases in temperature and UVB radiation

BMC Microbiology

Thierry Bouvier⁵  Helene Montanié³  Eric Fouilland⁵  Emilie LeFloc’h⁵  Amy Kirkham¹  Behzad Mostajir⁵  Francesca Vidussi⁵  Jean Pascal Torréton⁵  Corinne Bouvier⁵  Yvan Bettarel⁵  Stephan Jacquet¹  Jean Francois Ghiglione²  Marc Bouvy⁵  Didier Debroas⁴  Cécile Lepère⁴  Isabelle Domaizon¹ 

[1] INRA, UMR 42 CARRTEL, 75 avenue de Corzent, BP511, Thonon-les-bains, F-74200, France;CNRS, UMR 7621, LOMIC, Observatoire Océanologique, Avenue de Fontaulé, Banyuls/mer, F-66651, France;LIttoral ENvironnement et Sociétés (LIENSs) - UMR 6250, Université de La Rochelle, Institut du Littoral et de l’Environnement (ILE), 2 rue Olympe de Gouges, La Rochelle, 17 000, France;Clermont Université, Université Blaise Pascal, CNRS, UMR 6023, LMGE, BP 10448, Avenue des Landais, Clermont Ferrand, F-63000, France;Université Montpellier 2, UMR 5119 ECOSYM, CNRS, IRD, Ifremer, Université Montpellier 1, Place E Bataillon, cc 093, Montpellier cedex 5, 34095, France

关键词: Mediterranean lagoon;    Microcosms experiment;    UVB radiation;    Temperature;    Molecular diversity;    Small eukaryotes;   

Others  :  1221762
DOI  :  10.1186/1471-2180-12-202

 received in 2012-03-22, accepted in 2012-08-27,  发布年份 2012

【 摘 要 】

Background

Small size eukaryotes play a fundamental role in the functioning of coastal ecosystems, however, the way in which these micro-organisms respond to combined effects of water temperature, UVB radiations (UVBR) and nutrient availability is still poorly investigated.

Results

We coupled molecular tools (18S rRNA gene sequencing and fingerprinting) with microscope-based identification and counting to experimentally investigate the short-term responses of small eukaryotes (<6 μm; from a coastal Mediterranean lagoon) to a warming treatment (+3°C) and UVB radiation increases (+20%) at two different nutrient levels. Interestingly, the increase in temperature resulted in higher pigmented eukaryotes abundances and in community structure changes clearly illustrated by molecular analyses. For most of the phylogenetic groups, some rearrangements occurred at the OTUs level even when their relative proportion (microscope counting) did not change significantly. Temperature explained almost 20% of the total variance of the small eukaryote community structure (while UVB explained only 8.4%). However, complex cumulative effects were detected. Some antagonistic or non additive effects were detected between temperature and nutrients, especially for Dinophyceae and Cryptophyceae.

Conclusions

This multifactorial experiment highlights the potential impacts, over short time scales, of changing environmental factors on the structure of various functional groups like small primary producers, parasites and saprotrophs which, in response, can modify energy flow in the planktonic food webs.

【 授权许可】

   
2012 Domaizon et al.; licensee BioMed Central Ltd.

【 图 表 】

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