Morus alba and active compound oxyresveratrol exert anti-inflammatory activity via inhibition of leukocyte migration involving MEK/ERK signaling

BMC Complementary and Alternative Medicine

Wen-Chin Yang⁶  Yea-Lih Lin¹  Clément Mettling¹  Den-Jen Wu⁵  Ran-Juh Wang⁵  Evangeline C Amor³  Francesca N Beltran³  Chun-Houh Chen⁴  Yin-Jing Tien⁴  Yi-Ching Chen² 

[1] Institut de Génétique Humaine (CNRS UPR-1142), Montpellier, France;Agricultural Biotechnology Research Center, Academia Sinica, 128, Sec. 2, Academia Road, Nankang, Taipei 11501, Taiwan;Institute of Chemistry, College of Science, University of the Philippines, Quezon City, Philippines;Institute of Statistical Science, Academia Sinica, Taipei, Taiwan;Miaoli District Agricultural Research and Extension Station, Council of Agriculture, Miaoli, Taiwan;Institute of Zoology, National Taiwan University, Taipei, 106, Taiwan

关键词: Phytochemistry and T-cells;    Morus;    CXCR4;    Chemotaxis;   

Others  :  1230947
DOI  :  10.1186/1472-6882-13-45

 received in 2012-11-10, accepted in 2013-02-12,  发布年份 2013

【 摘 要 】

Background

Morus alba has long been used in traditional Chinese medicine to treat inflammatory diseases; however, the scientific basis for such usage and the mechanism of action are not well understood. This study investigated the action of M. alba on leukocyte migration, one key step in inflammation.

Methods

Gas chromatography-mass spectrometry (GC-MS) and cluster analyses of supercritical CO₂ extracts of three Morus species were performed for chemotaxonomy-aided plant authentication. Phytochemistry and CXCR4-mediated chemotaxis assays were used to characterize the chemical and biological properties of M. alba and its active compound, oxyresveratrol. fluorescence-activated cell sorting (FACS) and Western blot analyses were conducted to determine the mode of action of oxyresveratrol.

Results

Chemotaxonomy was used to help authenticate M. alba. Chemotaxis-based isolation identified oxyresveratrol as an active component in M. alba. Phytochemical and chemotaxis assays showed that the crude extract, ethyl acetate fraction and oxyresveratrol from M. alba suppressed cell migration of Jurkat T cells in response to SDF-1. Mechanistic study indicated that oxyresveratrol diminished CXCR4-mediated T-cell migration via inhibition of the MEK/ERK signaling cascade.

Conclusions

A combination of GC-MS and cluster analysis techniques are applicable for authentication of the Morus species. Anti-inflammatory benefits of M. alba and its active compound, oxyresveratrol, may involve the inhibition of CXCR-4-mediated chemotaxis and MEK/ERK pathway in T and other immune cells.

【 授权许可】

   
2013 Chen et al.; licensee BioMed Central Ltd.

【 图 表 】

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