Antioxidant, genotoxic and antigenotoxic activities of daphne gnidium leaf extracts

BMC Complementary and Alternative Medicine

Leila Chekir-Ghedira²  Kamel Ghedira¹  Somaya Kilani-Jaziri¹  Aicha Neffati¹  Amira Loussaif¹  Jihed Boubaker¹  Fadwa Chaabane¹ 

[1] Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03, Faculté de Pharmacie de Monastir, Monastir, 5000, Tunisie;Laboratoire de Biologie Cellulaire et Moléculaire, Faculté de Médicine Dentaire, de Monastir, Rue Avicenne, 5000, Tunisie

关键词: Antigenotoxic;    Antioxidant;    Daphne gnidium;   

Others  :  1232015
DOI  :  10.1186/1472-6882-12-153

 received in 2012-04-19, accepted in 2012-09-08,  发布年份 2012

【 摘 要 】

Background

Plants play a significant role in maintaining human health and improving the quality of human life. They serve humans well as valuable components of food, as well as in cosmetics, dyes, and medicines. In fact, many plant extracts prepared from plants have been shown to exert biological activity in vitro and in vivo. The present study explored antioxidant and antigenotoxic effects of Daphne gnidium leaf extracts.

Methods

The genotoxic potential of petroleum ether, chloroform, ethyl acetate, methanol and total oligomer flavonoid (TOF) enriched extracts from leaves of Daphne gnidium, was assessed using Escherichia coli PQ37. Likewise, the antigenotoxicity of the same extracts was tested using the “SOS chromotest test”. Antioxidant activities were studied using non enzymatic and enzymatic method: NBT/Riboflavine and xantine oxidase.

Results

None of the different extracts produced a genotoxic effect, except TOF extract at the lowest tested dose. Our results showed that D. gnidium leaf extracts possess an antigenotoxic effect against the nitrofurantoin a mutagen of reference. Ethyl acetate and TOF extracts were the most effective in inhibiting xanthine oxidase activity. While, methanol extract was the most potent superoxide scavenger when tested with the NBT/Riboflavine assay.

Conclusions

The present study has demonstrated that D. gnidium leaf extract possess antioxidant and antigenotoxic effects. These activities could be ascribed to compounds like polyphenols and flavonoid. Further studies are required to isolate the active molecules.

【 授权许可】

   
2012 Chaabane et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1 .

Figure 2 .

Figure 3 .

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