The Trip Adviser guide to the protein science world: a proposal to improve the awareness concerning the quality of recombinant proteins

BMC Research Notes

Ario de Marco¹  Tsafi Danieli²  Mario Lebendiker² 

[1] Department of Biomedical Sciences and Engineering, University of Nova Gorica, Glavni Trg 9, SI-5261 Vipava, Slovenia;Protein Expression and Purification Facilities, Wolfson Centre for Applied Structural Biology, Givat-Ram Campus - The Hebrew University of Jerusalem, 91904 Jerusalem, Israel

关键词: Protein quality;    Data reproducibility;    Data annotation;   

Others  :  1129999
DOI  :  10.1186/1756-0500-7-585

 received in 2014-03-26, accepted in 2014-07-31,  发布年份 2014

【 摘 要 】

In many research articles, where protein purification is required for various assays, (protein-protein interactions, activity assays, etc.), we always have access to the final results, but seldom have access to the raw data required for an accurate evaluation of the protein quality. This data is extremely important on one hand to critically evaluate the quality of the proteins used in the described research and, on the other hand, to allow other laboratories to safely use the described procedure in a reproducible manner. We herby propose to include a standardized methodology that can easily be incorporated in research papers. Moreover, this methodology can be utilized as a “quality control” ladder, where the more information given, will lead to a higher ranking of the article. This “quality control” stamp will allow researchers retrieving relevant and useful materials and methods in the field of protein research.

【 授权许可】

   
2014 Lebendiker et al.; licensee BioMed Central Ltd.

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