Assessment of maximal lactate steady state during treadmill exercise in SHR

BMC Research Notes

Octávio Luiz Franco²  Rinaldo Wellerson Pereira²  Clarissa Pedrosa da Costa Gomes¹  Bernardo de Assis Petriz¹  Jeeser Alves Almeida² 

[1] Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SGAN, Quadra 916, Módulo B, Av. W5 Norte, Brasília, DF, 70790-160, Brazil;Programa de Pós-Graduação em Educação Física, Universidade Católica de Brasília, Brasília, DF, Brazil

关键词: Treadmill Testing;    Lactate;    MLSS;    Aerobic Exercise;    SHR;    Hypertension;   

Others  :  1165095
DOI  :  10.1186/1756-0500-5-661

 received in 2012-08-22, accepted in 2012-11-26,  发布年份 2012

【 摘 要 】

Background

Spontaneously hypertensive rats (SHR) are one of the main animal models used for studying the effects of exercise on hypertension. Therefore, the determination of adequate intensity has been essential for secure and optimized exercise prescriptions concerning hypertensive subjects. This study aimed to identify the MLSS in SHR by using a treadmill test to improve the protocols and further prescriptions of exercise intensity.

Findings

In order to carry out this determination, SHR (n = 10) animals (~17.5 weeks; 227.4 ± 29.3 g; 172.4 ± 8.1 mmHg systolic blood pressure) were divided into two groups (G1 n = 5; G2 n = 5). Rats underwent a test with three different velocities to determine the MLSS. The MLSS was considered as the highest effort intensity where the blood lactate did not vary more than 1 mmol.L^-1 from the 10^th to the 25^th minute. The MLSS was reached at a velocity of 20 m.min^-1 with 3.8 ± 0.5 mmol.L^-1 of lactate for G1. Additionally, the results were validated in G2. However, when the test was applied at 25 m.min^-1, there was no stabilization of BLC in G1 and G2.

Conclusions

In this study it was possible to identify the MLSS in SHR rats, which is an excellent evaluation tool to control exercise intensity. These data are of considerable importance in studies using physical exercise as a means of research in hypertension and may lead to the intensity of exercise being prescribed more appropriately.

【 授权许可】

   
2012 Almeida et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

【 参考文献 】

• [1]Sliwa K, Stewart S, Gersh BJ: Hypertension: a global perspective. Circulation 2011, 123:2892-2896.
• [2]Oparil S, Zaman MA, Calhoun DA: Pathogenesis of hypertension. Ann Intern Med 2003, 139:761-776.
• [3]MacDonald JR: Potential causes, mechanisms, and implications of post exercise hypotension. J Hum Hypertens 2002, 16:225-236.
• [4]Fitzgerald W: Labile hypertension and jogging: new diagnostic tool or spurious discovery? Br Med J (Clin Res Ed) 1981, 282:542-544.
• [5]Anunciação PG, Polito MD: A Review on Post-exercise Hypotension in Hypertensive Individual. Arq Bras Cardiol 2011, 96:425-426.
• [6]Kolwicz SC, MacDonnell SM, Renna BF, Reger PO, Seqqat R, Rafiq K, Kendrick ZV, Houser SR, Sabri A, Libonati JR: Left ventricular remodeling with exercise in hypertension. Am J Physiol Heart Circ Physiol 2009, 297:H1361-H1368.
• [7]Beneke R, Leithauser RM, Ochentel O: Blood lactate diagnostics in exercise testing and training. Int J Sports Physiol Perform 2011, 6:8-24.
• [8]Beneke R, von Duvillard SP: Determination of maximal lactate steady state response in selected sports events. Med Sci Sports Exerc 1996, 28:241-246.
• [9]Billat VL, Sirvent P, Py G, Koralsztein JP, Mercier J: The concept of maximal lactate steady state: a bridge between biochemistry, physiology and sport science. Sports Med 2003, 33:407-426.
• [10]Lindner AE: Maximal lactate steady state during exercise in blood of horses. J Anim Sci 2010, 88:2038-2044.
• [11]Ferreira JC, Rolim NP, Bartholomeu JB, Gobatto CA, Kokubun E, Brum PC: Maximal lactate steady state in running mice: effect of exercise training. Clin Exp Pharmacol Physiol 2007, 34:760-765.
• [12]Contarteze RV, Manchado FB, Gobatto CA, De Mello MA: Stress biomarkers in rats submitted to swimming and treadmill running exercises. Comp Biochem Physiol A Mol Integr Physiol 2008, 151:415-422.
• [13]Manchado FB, Gobatto CA, Contartaze RVL, Papoti M, Mello MAR: Maximal lactate steady state in running rats. J Exerc Physiol 2005, 8:29-35.
• [14]Cunha RR, Cunha VN, Segundo PR, Moreira SR, Kokubun E, Campbell CS, de Oliveira RJ, Simoes HG: Determination of the lactate threshold and maximal blood lactate steady state intensity in aged rats. Cell Biochem Funct 2009, 27:351-357.
• [15]Harriss DJ, Atkinson G: Update–Ethical standards in sport and exercise science research. Int J Sports Med 2011, 32:819-821.
• [16]Rodrigues B, Figueroa DM, Mostarda CT, Heeren MV, Irigoyen MC, De Angelis K: Maximal exercise test is a useful method for physical capacity and oxygen consumption determination in streptozotocin-diabetic rats. Cardiovasc Diabetol 2007, 6:38. BioMed Central Full Text
• [17]Wallace JP: Exercise in hypertension. A clinical review. Sports Med 2003, 33:585-598.
• [18]Mach C, Foster C, Brice G, Mikat RP, Porcari JP: Effect of exercise duration on postexercise hypotension. J Cardiopulm Rehabil 2005, 25:366-369.
• [19]Jones H, George K, Edwards B, Atkinson G: Is the magnitude of acute post-exercise hypotension mediated by exercise intensity or total work done. Eur J Appl Physiol 2007, 102:33-40.
• [20]Morais PK, Campbell CS, Sales MM, Motta DF, Moreira SR, Cunha VN, Benford RE, Simões HG: Acute resistance exercise is more effective than aerobic exercise for 24 h blood pressure control in type 2 diabetics. Diabetes Metab 2011, 37:112-117.
• [21]Lee SK, Kim CS, Kim HS, Cho EJ, Joo HK, Lee JY, Lee EJ, Park JB, Jeon BH: Endothelial nitric oxide synthase activation contributes to post-exercise hypotension in spontaneously hypertensive rats. Biochem Biophys Res Commun 2009, 382:711-714.
• [22]Garciarena CD, Pinilla OA, Nolly MB, Laguens RP, Escudero EM, Cingolani HE, Ennis IL: Endurance training in the spontaneously hypertensive rat: conversion of pathological into physiological cardiac hypertrophy. Hypertension 2009, 53:708-714.
• [23]Melo RM, Martinho E Jr, Michelini LC: Training-induced, pressure-lowering effect in SHR: wide effects on circulatory profile of exercised and nonexercised muscles. Hypertension 2003, 42:851-857.
• [24]Sun MW, Qian FL, Wang J, Tao T, Guo J, Wang L, Lu AY, Chen H: Low-intensity voluntary running lowers blood pressure with simultaneous improvement in endothelium-dependent vasodilatation and insulin sensitivity in aged spontaneously hypertensive rats. Hypertens Res 2008, 31:543-552.
• [25]Pardono E, Sotero Rda C, Hiyane W, Mota MR, Campbell CS, Nakamura FY, Simoes HG: Maximal lactate steady-state prediction through quadratic modeling of selected stages of the lactate minimum test. J Strength Cond Res 2008, 22:1073-1080.
• [26]Ceroni A, Chaar LJ, Bombein RL, Michelini LC: Chronic absence of baroreceptor inputs prevents training-induced cardiovascular adjustments in normotensive and spontaneously hypertensive rats. Exp Physiol 2009, 94:630-640.
• [27]Felix JV, Michelini LC: Training-induced pressure fall in spontaneously hypertensive rats is associated with reduced angiotensinogen mRNA expression within the nucleus tractus solitarii. Hypertension 2007, 50:780-785.