Mapping QTL for the traits associated with heat tolerance in wheat (Triticum aestivum L.)

BMC Genetics

Allan Fritz²  Robert Bowden³  Pagadala Venkata Vara Prasad²  Jesse Poland⁴  Kolluru Vijayalakshmi²  Md Ali Babar¹  Shyamal Krishna Talukder² 

[1] Department of Agronomy, University of Florida, Gainesville, Florida, USA;Department of Agronomy, Kansas State University, Manhattan 66506, KS, USA;USDA/ARS/Hard Winter Wheat Genetics Research Unit, Kansas State University, Manhattan 66506, KS, USA;Department of Plant Pathology, Kansas State University, Manhattan 66506, KS, USA

关键词: Plasmamembrane damage;    Thylakoid membrane damage;    GBS-SNP;    Heat tolerance;    Wheat;   

Others  :  1085315
DOI  :  10.1186/s12863-014-0097-4

 received in 2014-05-27, accepted in 2014-08-29,  发布年份 2014

【 摘 要 】

Background

High temperature (heat) stress during grain filling is a major problem in most of the wheat growing areas. Developing heat tolerant cultivars has become a principal breeding goal in the Southern and Central Great Plain areas of the USA. Traits associated with high temperature tolerance can be used to develop heat tolerant cultivars in wheat. The present study was conducted to identify chromosomal regions associated with thylakoid membrane damage (TMD), plasmamembrane damage (PMD), and SPAD chlorophyll content (SCC), which are indicative of high temperature tolerance.

Results

In this study we have reported one of the first linkage maps in wheat using genotype by sequencing SNP (GBS-SNP) markers to extreme response to post anthesis heat stress conditions. The linkage map was comprised of 972 molecular markers (538 Bin, 258 AFLPs, 175 SSRs, and an EST). The genotypes of the RIL population showed strong variation for TMD, SCC and PMD in both generations (F₁₀ and F₉). Composite interval mapping identified five QTL regions significantly associated with response to heat stress. Associations were identified for PMD on chromosomes 7A, 2B and 1D, SCC on 6A, 7A, 1B and 1D and TMD on 6A, 7A and 1D. The variability (R²) explained by these QTL ranged from 11.9 to 30.6% for TMD, 11.4 to 30.8% for SCC, and 10.5 to 33.5% for PMD. Molecular markers Xbarc113 and AFLP AGCTCG-347 on chromosome 6A, Xbarc121 and Xbarc49 on 7A, gwm18 and Bin1130 on 1B, Bin178 and Bin81 on 2B and Bin747 and Bin1546 on 1D were associated with these QTL.

Conclusion

The identified QTL can be used for marker assisted selection in breeding wheat for improved heat tolerance in Ventnor or Karl 92 genetic background.

【 授权许可】

   
2014 Talukder et al.; licensee BioMed Central Ltd.

【 图 表 】

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