Fatty acid profiles and desaturase-encoding genes are different in thermo- and psychrotolerant strains of the Bacillus cereus Group

BMC Research Notes

Julien Brillard⁴  Véronique Broussolle³  Christophe Nguyen-the³  Benoit De Sarrau²  Marie-Hélène Guinebretière¹  Sara Esther Diomandé³ 

[1] INRA, UMR408 SQPOV, Site Agroparcs, 228 route de l’Aérodrome, Avignon Cedex 9, 84914, France;Xurian Environnement, ZAE Béziers Ouest, rue du Jéroboam, Béziers, 34500, France;Université d’Avignon, UMR408 Sécurité et Qualité des Produits d’Origine Végétale, Avignon, 84000, France;INRA, UMR 1333 DGIMI, Université Montpellier, Montpellier Cedex 5, 34095, France

关键词: Temperature tolerance;    Desaturases;    Fatty acids;    B. cereus Group;   

Others  :  1230908
DOI  :  10.1186/s13104-015-1288-4

 received in 2014-11-14, accepted in 2015-07-22,  发布年份 2015

【 摘 要 】

Background

The Bacillus cereus Group consists of closely-related bacteria, including pathogenic or harmless strains, and whose species can be positioned along the seven phylogenetic groups of Guinebretière et al. (I–VII). They exhibit different growth-temperature ranges, through thermotolerant to psychrotolerant thermotypes. Among these, B. cytotoxicus is an atypical thermotolerant and food-poisoning agent affiliated to group VII whose thermotolerance contrasts with the mesophilic and psychrotolerant thermotypes associated to the remaining groups I–VI. To understand the role of fatty acid (FA) composition in these variable thermotypes (i.e. growth behavior vs temperatures), we report specific features differentiating the FA pattern of B. cytotoxicus (group VII) from its counterparts (groups I–VI).

Findings

The FA pattern of thermotolerant group VII (B. cytotoxicus) displayed several specific features. Most notably, we identified a high ratio of the branched-chain FAs iso-C15/iso-C13 (i15/i13) and the absence of the unsaturated FA (UFA) C16:1(5) consistent with the absence of ∆5 desaturase DesA. Conversely, phylogenetic groups II–VI were characterized by lower i15/i13 ratios and variable proportions of C16:1(5) depending on thermotype, and presence of the DesA desaturase. In mesophilic group I, thermotype seemed to be related to an atypically high amount of C16:1(10) that may involve ∆10 desaturase DesB.

Conclusion

The levels of i15/i13 ratio, C16:1(5) and C16:1(10) UFAs were related to growth temperature variations recorded between thermotypes and/or phylogenetic groups. These FA are likely to play a role in membrane fluidity and may account for the differences in temperature tolerance observed in B. cereus Group strains.

【 授权许可】

   
2015 Diomandé et al.

【 图 表 】

Fig.1.

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