Non-thermal plasma treatment altered gene expression profiling in non-small-cell lung cancer A549 cells

BMC Genomics

Wei Han⁴  Lingzhi Bao⁴  Cheng Cheng¹  Wei Li³  K. N. Yu²  Jie Ma⁵  Jue Hou³ 

[1] Institute of Plasma Physics, Hefei Institutes of Physical Sciences, Chinese Academy of Sciences, Hefei, China;Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong;Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China;350 Shushanhu Road, Hefei 230031, Anhui, P. R. China;School of Life Sciences, University of Science and Technology of China, Hefei, China

关键词: Multiple signal pathways;    NSCLC A549 cell line;    Gene profiling;    Non-thermal plasma;   

Others  :  1216242
DOI  :  10.1186/s12864-015-1644-8

 received in 2014-12-03, accepted in 2015-05-20,  发布年份 2015

【 摘 要 】

Background

Recent technological advances in atmospheric plasmas have made the creation of non-thermal atmospheric pressure plasma (NTP) possible for utilization in the medical field. Although accumulated evidence suggests that NTP induces cell death in various cancer cell types thus offering a promising alternative treatment strategy, the mechanism underlying its therapeutic effect is not fully understood.

Results

We analyzed relevant signaling cascades associated with the tumor protein p53, in particular the cell cycle arrest, DNA damage as well as the underlying apoptosis pathways. Based on our results, the major effect from plasma exposure was found to be the activation of MAPK and p53 signaling pathways, resulting in changes in gene expression of MEKK, GADD, FOS and JUN. Finally, a significant modulation in expression of genes related to cellular proliferation and differentiation was observed.

Conclusion

Overall, the presented data of the tumor transcriptome helped identify the key players in modulated gene expression following exposure to plasma at the molecular level, and also helped interpret the downstream processes. The present work laid the foundation for further studies to clarify the roles of multiple pathways in plasma-induced biological processes. Further investigation of these genes in other cell lines may reveal comprehensive mechanisms of plasma induced effects.

【 授权许可】

   
2015 Hou et al.

【 图 表 】

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Figure 1.

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