Development and characterization of microsatellite loci for common raven (Corvus corax) and cross species amplification in other Corvidae

BMC Research Notes

Kevin Winker²  Brant Faircloth⁴  Travis Glenn⁵  Stacey L. Lance³  Cory Spern¹  Tianyu Li¹  Leping Wan¹  Christin L. Pruett¹ 

[1] Department of Biological Sciences, Florida Institute of Technology, Melbourne 32901, FL, USA;University of Alaska Museum, University of Alaska Fairbanks, Fairbanks 99775, AK, USA;Savannah River Ecology Laboratory, University of Georgia, Aiken 29802, SC, USA;Department of Biological Sciences, Louisiana State University, Baton Rouge 70803, LA, USA;Department of Environmental Health Science, University of Georgia, Athens 30602, GA, USA

关键词: Alaska;    Holarctic;    Microsatellite;    PCR primers;    Corvus;   

Others  :  1232739
DOI  :  10.1186/s13104-015-1643-5

 received in 2015-07-17, accepted in 2015-10-27,  发布年份 2015

【 摘 要 】

Background

A priority for conservation is the identification of endemic populations. We developed microsatellite markers for common raven (Corvus corax), a bird species with a Holarctic distribution, to identify and assess endemic populations in Alaska.

Results

From a total of 50 microsatellite loci, we isolated and characterized 15 loci. Eight of these loci were polymorphic and readily scoreable. Eighteen to 20 common ravens from Fairbanks, Alaska were genotyped showing the following variability: 3–8 alleles per locus, 0.25–0.80 observed heterozygosity (H _o ), and 0.30–0.80 expected heterozygosity (H _e ). All loci were in Hardy–Weinberg equilibrium and linkage equilibrium and many loci amplified and were polymorphic in related taxa.

Conclusions

These loci will be used to identify endemic populations of common raven and assess their genetic diversity and connectivity.

【 授权许可】

   
2015 Pruett et al.

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