Dose-dependent inhibition of gastric injury by hydrogen in alkaline electrolyzed drinking water

BMC Complementary and Alternative Medicine

Yun Lu³  Wenjun Liu³  Natalia Velasquez¹  Lingyan Hou¹  Yasuhiro Saihara²  Yoshinori Tanaka²  Guodong Shang¹  Jinling Xue¹ 

[1] State Key Joint Laboratory of Environmental Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, P.R. China;Panasonic Corporation, Appliances Company, 2-3-1-2 Noji-Higashi, Kusatsu-city, Shiga 525-8502, Japan;State Environmental Protection Key Laboratory of Microorganism Application and Risk Control, Tsinghua University, Beijing 100084, P.R. China

关键词: Oxidative stress;    Hydrogen;    Gastric injury;    Dose–response;    Alkaline electrolyzed water;   

Others  :  1220242
DOI  :  10.1186/1472-6882-14-81

 received in 2013-08-13, accepted in 2014-02-25,  发布年份 2014

【 摘 要 】

Background

Hydrogen has been reported to relieve damage in many disease models, and is a potential additive in drinking water to provide protective effects for patients as several clinical studies revealed. However, the absence of a dose–response relationship in the application of hydrogen is puzzling. We attempted to identify the dose–response relationship of hydrogen in alkaline electrolyzed drinking water through the aspirin induced gastric injury model.

Methods

In this study, hydrogen-rich alkaline water was obtained by adding H₂ to electrolyzed water at one atmosphere pressure. After 2 weeks of drinking, we detected the gastric mucosal damage together with MPO, MDA and 8-OHdG in rat aspirin induced gastric injury model.

Results

Hydrogen-dose dependent inhibition was observed in stomach mucosal. Under pH 8.5, 0.07, 0.22 and 0.84 ppm hydrogen exhibited a high correlation with inhibitory effects showed by erosion area, MPO activity and MDA content in the stomach. Gastric histology also demonstrated the inhibition of damage by hydrogen-rich alkaline water. However, 8-OHdG level in serum did not have significant hydrogen-dose dependent effect. pH 9.5 showed higher but not significant inhibitory response compared with pH 8.5.

Conclusions

Hydrogen is effective in relieving the gastric injury induced by aspirin-HCl, and the inhibitory effect is dose-dependent. The reason behind this may be that hydrogen-rich water directly interacted with the target tissue, while the hydrogen concentration in blood was buffered by liver glycogen, evoking a suppressed dose–response effect. Drinking hydrogen-rich water may protect healthy individuals from gastric damage caused by oxidative stress.

【 授权许可】

   
2014 Xue et al.; licensee BioMed Central Ltd.

【 图 表 】

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