Cryptosporidium andersoni as a novel predominant Cryptosporidium species in outpatients with diarrhea in Jiangsu Province, China

BMC Infectious Diseases

Yujuan Shen²  Yijin Lin¹  Jianping Cao²  Wei Pan²  Lei Chu¹  Hua Liu²  Hong Zhao¹  Aiqin Liu³  Zhongying Yuan²  Jinhua Ren¹  Yanyan Jiang² 

[1] Department of Infectious Diseases, Danyang Hospital of Jiangsu Province, Danyang 212300, People’s Republic of China;National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Key Laboratory of Parasite and Vector Biology, Ministry of Health; WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, People’s Republic of China;Department of Parasitology, Harbin Medical University, Harbin 150081, People’s Republic of China

关键词: Prevalence;    Predominant species;    Stool specimens;    Diarrhea;    Cryptosporidium andersoni;   

Others  :  1125406
DOI  :  10.1186/s12879-014-0555-7

 received in 2014-06-16, accepted in 2014-10-10,  发布年份 2014

【 摘 要 】

Background

Cryptosporidium hominis and C. parvum are usually considered to be the major pathogens responsible for human cryptosporidiosis. However, there have been few studies regarding the molecular epidemiology of Cryptosporidium in human infections in China. Here we investigated Cryptosporidium infection in patients with diarrhea, in Danyang Hospital of Jiangsu Province, China, at the genotype level.

Methods

A total of 232 stool specimens were collected from outpatients with diarrhea in Danyang Hospital of Jiangsu Province, China, from February 2012 to January 2013. Each specimen was stained from direct fecal smears and examined for Cryptosporidium using modified acid fast staining and microscopy. Moreover, genomic DNA of each fecal sample was screened for the presence of Cryptosporidium with nested PCR, which was genotyped by analyzing the DNA sequences of small subunit rRNA (SSU rRNA).

Results

The average infection rate of Cryptosporidium was 1.3% (3/232) by microscopy and subjected to PCR amplification of the SSU rRNA gene of Cryptosporidium, with 9.91% (23/232) being positive for Cryptosporidium with a significant peak in autumn. Based on the SSU rRNA gene, two Cryptosporidium spp. were identified, including C. andersoni (n =21) and C. hominis (n =2). Two types of C. andersoni, designated as A₃₇₀⁺ and A₃₇₀^- , were found in the SSU rRNA gene in our present study, which was 100% homologous to C. andersoni infections derived from dairy calves and goats, respectively. The clinical questionnaires showed no significant difference in age, gender and frequency of diarrhea, but duration of diarrhea was shorter for C. andersoni than that of C. hominis (mean, 2 vs. 4 days; p <0.01).

Conclusions

C. andersoni is the dominant species in Danyang City of Jiangsu Province. The fact that SSU rRNA sequences of C. andersoni obtained from human stools exhibited 100% homologous to those derived from dairy calves and goats supported that C. andersoni infection might be attributable to animal origin. The difference in the duration of diarrhea of C. andersoni and C. hominis indicated that different Cryptosporidium species might cause different clinical manifestations.

【 授权许可】

   
2014 Jiang et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 2.

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