IL-17-producing peripheral blood CD177+ neutrophils increase in allergic asthmatic subjects

Allergy, Asthma & Clinical Immunology

Carlos Ramirez-Velazquez³  Elena Cristina Castillo¹  Leopoldo Guido-Bayardo²  Vianney Ortiz-Navarrete¹ 

[1] Molecular Biomedicine Department, Centro de Investigación y de Estudios Avanzados (CINVESTAV)-IPN, Av. IPN No. 2508, Colonia San Pedro Zacatenco, México, DF CP. 07360, México

[2] Allergy Department, Centro Médico Nacional 20 de Noviembre ISSSTE, Felix Cuevas 540, Colonia del Valle, Mexico, DF CP 03229, Mexico

[3] Allergy Department, Hospital General Dr. Fernando Quiroz Gutiérrez, ISSSTE, Calle Felipe Angeles y Canario. Colonia Bellavista, Mexico, DF CP 01140, Mexico

关键词: Blood;    Allergic asthma;    IL-17;    Neutrophils;   

Others  :  792233
DOI  :  10.1186/1710-1492-9-23

 received in 2013-02-27, accepted in 2013-06-30,  发布年份 2013

【 摘 要 】

Background

A T helper cell (T_H) 17-biased response has been observed in patients with allergic asthma, particularly in those with neutrophil accumulation in the lung. Therefore, we sought to test the hypothesis that neutrophils might be an important source of interleukin (IL)-17 in allergic asthma.

Methods

Whole peripheral blood cells from non-asthmatic control subjects (n = 17) and patients with mild asthma (n = 7), moderate but persistent asthma (n = 4), or acute asthma (n = 6) were analyzed for IL-17A expression in CD177⁺ neutrophils. IL-17A expression was also analyzed in CD3⁺CD4⁺ and CD3⁺CD8⁺ lymphocyte populations. Asthmatic patients were classified as allergic to fungi, indoor allergens, or other allergens (e.g., pollen) based on a positive intradermal allergy test reaction.

Results

The percentage of CD177⁺ neutrophils in whole blood of asthmatic patients was higher than in healthy controls and highest in the moderate asthma group. Furthermore, the percentage of CD177⁺IL-17⁺ neutrophils was elevated in patients with mild asthma, whereas the CD4⁺ IL-17⁺ lymphocyte population was higher in asthmatic patients and highest in those with moderate but persistent asthma. We also found that the four patients that were allergic to fungi had the highest percentage of CD177⁺IL17⁺ neutrophils and CD8⁺IL17⁺ lymphocytes.

Conclusion

IL17⁺CD177⁺ Neutrophils increase in allergic asthma patients especially when allergic to fungi. This cell population, through release of IL-17, might be contributing during the initial phase asthmatic disease and/or during disease progression but its role has not yet been established.

【 授权许可】

   
2013 Ramirez-Velazquez et al.; licensee BioMed Central Ltd.

【 图 表 】

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