Reference values for amino acids and acylcarnitines in peripheral blood in Quarter horses and American Miniature horses

Acta Veterinaria Scandinavica

Irám Pablo Rodríguez-Sánchez²  Víctor Manuel Treviño-Alvarado¹  María del Rosario Torres-Sepúlveda²  Liliana Aracely López-Saldaña⁵  Gustavo Ponce-García⁴  Graciela Areli López-Uriarte²  María del Consuelo Ruiz-Herrera²  Diana Elisa Zamora-Ávila⁵  Jesús Zacarías Villarreal-Pérez³  Guillermo Dávalos-Aranda⁵  Laura Elia Martínez-de-Villarreal² 

[1] Tecnológico de Monterrey Cátedra de Bioinformática, Campus Monterrey, Monterrey, Nuevo León, Mexico

[2] Departamento de Genética, Hospital Universitario “Dr. José Eleuterio González”, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico

[3] Servicio de Endocrinología, Hospital Universitario “Dr. José Eleuterio González”, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico

[4] Departamento de Zoología de invertebrados, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico

[5] Departamento de Genética, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico

关键词: Tandem mass spectrometry;    Quarter horse;    Miniature horse;    Metabolic profile;    Acylcarnitine;    Amino acid;   

Others  :  1228609
DOI  :  10.1186/s13028-015-0144-9

 received in 2015-02-24, accepted in 2015-09-14,  发布年份 2015

【 摘 要 】

Background

Free amino acids and acylcarnitines circulating in the blood can be used for diagnosis for metabolic illness and imbalances. To date, the normal reference ranges of amino acids and acylcarnitines in horse peripheral blood have not been established. In this study, the concentrations of 12 amino acids and 26 acylcarnitines were determined by tandem mass spectrometry in complete blood from 100 healthy horses (50 Quarter horses (QH) [23 males and 27 females] and 50 American Miniature horses (AMH) [15 males and 35 females]) with no signs of metabolic disease. The means and standard deviations were determined and data statistically analyzed.

Findings

Concentrations of short, medium, and long chain acylcarnitines were significantly higher in male AMH than in male QH. The concentrations of the amino acids alanine, arginine, glycine, proline (glycogenic), and leucine (ketogenic) were higher in the QH than in the AMH. Female AMH had higher concentrations of propionylcarnitine, leucine, proline, arginine, and ornithine than female QH.

Conclusions

Normal reference ranges of amino acids and acylcarnitines were established for AMH and QH. Significant differences were found in concentration of these compounds between breeds and gender.

【 授权许可】

   
2015 Rodríguez-Sánchez et al.

【 图 表 】

Fig.1.

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