Ingestion of milk containing the Dp2 peptide, a dust mite allergen, protects mice from allergic airway inflammation and hyper-responsiveness

Allergy, Asthma & Clinical Immunology

Hsu-Chung Liu²  Shun-Yuan Pai¹  Winston TK Cheng⁵  Hsiao-Ling Chen⁴  Tung-Chou Tsai¹  Shang-Hsun Yang³  Chuan-Mu Chen⁶ 

[1] Department of Life Sciences, Agricultural Biotechnology Center, National Chung Hsing University, Kuo Kuang Rd, Taichung, 402, Taiwan

[2] Division of Chest Medicine, Department of Internal Medicine, Chung Shan Medical University Hospital and School of Medicine, Chung Shan Medical University, Taichung, Taiwan

[3] Department of Physiology, National Cheng Kung University, Tainan, Taiwan

[4] Department of Bioresources, Da-Yeh University, Changhwa, Taiwan

[5] Department of Animal Science and Biotechnology, Tunghai University, Taichung, Taiwan

[6] iEGG Center, National Chung Hsing University, Taichung, Taiwan

关键词: Tolerance;    Group 2 allergen of Dermatophagoides pteronyssinus;    Immunotherapy;    Asthma;    Allergen;    Transgenic mice;   

Others  :  792256
DOI  :  10.1186/1710-1492-9-21

 received in 2013-04-14, accepted in 2013-06-05,  发布年份 2013

【 摘 要 】

Background

Allergen-specific immunotherapy has been demonstrated to have potential for the treatment of allergic diseases. Transgenic animals are currently the best available bioreactors to produce recombinant proteins, which can be secreted in milk. It has not been clearly demonstrated whether milk from transgenic animals expressing recombinant allergens has immunomodulatory effects on allergic asthma.

Methods

We aimed to determine whether the oral administration of milk containing a mite allergen can down-regulate allergen-specific airway inflammation. Transgenic CD-1 mice that express a recombinant group 2 allergen from Dermatophagoides pteronyssinus (Dp2) in their milk were generated using an embryonic gene-microinjection technique. Mouse pups were fed transgenic Dp2-containing milk or wild-type milk. Subsequently, these mice were sensitized and challenged with Dp2 to induce allergic airway inflammation.

Results

Upon sensitization and challenge, mice fed transgenic Dp2 milk had decreased T-helper 2 (Th2) and increased T-helper 1 (Th1) responses in the airway compared with mice fed wild-type milk. Moreover, pre-treatment with transgenic Dp2 milk attenuated airway inflammation and decreased airway hyper-responsiveness.

Conclusions

This study provides new evidence that oral administration of transgenic milk containing the Dp2 allergen down-regulated and moderately protected against allergic airway inflammation. Milk from transgenic animals expressing allergens may have potential use in the prevention of allergic asthma.

【 授权许可】

   
2013 Liu et al.; licensee BioMed Central Ltd.

【 图 表 】

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