期刊论文详细信息
Biotechnology for Biofuels
Utilization of household food waste for the production of ethanol at high dry material content
Leonidas Matsakas2  Dimitris Kekos3  Maria Loizidou1  Paul Christakopoulos2 
[1] Unit of Environmental Science and Technology, School of Chemical Engineering, National Technical University of Athens, 5, Iroon Polytechniou Str, Zografou Campus, 15780 Athens, Greece
[2] Department of Civil, Biochemical and Chemical Process Engineering, Division of Sustainable Process Engineering, Environmental and Natural Resources Engineering, Luleå University of Technology, SE 971 87 Luleå, Sweden
[3] Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15780 Athens, Greece
关键词: Saccharomyces cerevisiae;    Subsequent fermentation;    Residue solids;    Household food waste;    Saccharification;    Liquefaction;    Ethanol;   
Others  :  794110
DOI  :  10.1186/1754-6834-7-4
 received in 2013-09-06, accepted in 2013-12-24,  发布年份 2014
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【 摘 要 】

Background

Environmental issues and shortage of fossil fuels have turned the public interest to the utilization of renewable, environmentally friendly fuels, such as ethanol. In order to minimize the competition between fuels and food production, researchers are focusing their efforts to the utilization of wastes and by-products as raw materials for the production of ethanol. household food wastes are being produced in great quantities in European Union and their handling can be a challenge. Moreover, their disposal can cause severe environmental issues (for example emission of greenhouse gasses). On the other hand, they contain significant amounts of sugars (both soluble and insoluble) and they can be used as raw material for the production of ethanol.

Results

Household food wastes were utilized as raw material for the production of ethanol at high dry material consistencies. A distinct liquefaction/saccharification step has been included to the process, which rapidly reduced the viscosity of the high solid content substrate, resulting in better mixing of the fermenting microorganism. This step had a positive effect in both ethanol production and productivity, leading to a significant increase in both values, which was up to 40.81% and 4.46 fold, respectively. Remaining solids (residue) after fermentation at 45% w/v dry material (which contained also the unhydrolyzed fraction of cellulose), were subjected to a hydrothermal pretreatment in order to be utilized as raw material for a subsequent ethanol fermentation. This led to an increase of 13.16% in the ethanol production levels achieving a final ethanol yield of 107.58 g/kg dry material.

Conclusions

In conclusion, the ability of utilizing household food waste for the production of ethanol at elevated dry material content has been demonstrated. A separate liquefaction/saccharification process can increase both ethanol production and productivity. Finally, subsequent fermentation of the remaining solids could lead to an increase of the overall ethanol production yield.

【 授权许可】

   
2014 Matsakas et al.; licensee BioMed Central Ltd.

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【 参考文献 】
  • [1]Li X, Kim TH, Nghiem NP: Bioethanol production from corn stover using aqueous ammonia pretreatment and two-phase simultaneous saccharification and fermentation (TPSSF). Bioresour Technol 2010, 101:5910-5916.
  • [2]Sarris D, Giannakis M, Philippoussis A, Komaitis M, Koutinas AA, Papanikolaou S: Conversions of olive mill wastewater-based media by Saccharomyces cerevisiae through sterile and non-sterile bioprocesses. J Chem Technol Biotechnol 2013, 88:958-969.
  • [3]Matsakas L, Christakopoulos P: Optimization of ethanol production from high dry matter liquefied dry sweet sorghum stalks. Biomass Bioenerg 2013, 51:91-98.
  • [4]Yan S, Chen X, Wu J, Wang P: Ethanol production from concentrated food waste hydrolysates with yeast cells immobilized on corn stalk. Appl Microbiol Biotechnol 2012, 94:829-838.
  • [5]Sims REH, Mabee W, Saddler JN, Taylor M: An overview of second generation biofuel technologies. Bioresour Technol 2010, 101:1570-1580.
  • [6]Moon HC, Song IS, Kim JC, Shirai Y, Lee DH, Kim JK, Chung SO, Kim DH, Oh KK, Cho YS: Enzymatic hydrolysis of food waste and ethanol fermentation. Int J Energ Res 2009, 33:164-172.
  • [7]Zhang M, Wang F, Su R, Qi W, He Z: Ethanol production from high dry matter corncob using fed-batch simultaneous saccharification and fermentation after combined pretreatment. Bioresour Technol 2010, 101:4959-4964.
  • [8]Silva VN, Arruda P, Felipe MA, Gonçalves A, Rocha GM: Fermentation of cellulosic hydrolysates obtained by enzymatic saccharification of sugarcane bagasse pretreated by hydrothermal processing. J Ind Microbiol Biotechnol 2011, 38:809-817.
  • [9]Díaz MJ, Cara C, Ruiz E, Romero I, Moya M, Castro E: Hydrothermal pre-treatment of rapeseed straw. Bioresour Technol 2010, 101:2428-2435.
  • [10]Pérez JA, Ballesteros I, Ballesteros M, Sáez F, Negro MJ, Manzanares P: Optimizing liquid hot water pretreatment conditions to enhance sugar recovery from wheat straw for fuel-ethanol production. Fuel 2008, 87:3640-3647.
  • [11]European Communities: EC preparatory study on food waste in the EU27. [http://ec.europa.eu/environment/eussd/pdf/bio_foodwaste_report.pdf webcite]
  • [12]Yan S, Li J, Chen X, Wu J, Wang P, Ye J, Yao J: Enzymatical hydrolysis of food waste and ethanol production from the hydrolysate. Renew Energ 2011, 36:1259-1265.
  • [13]Lin CSK, Pfaltzgraff LA, Herrero-Davila L, Mubofu EB, Abderrahim S, Clark JH, Koutinas AA, Kopsahelis N, Stamatelatou K, Dickson F, Thankappan S, Mohamed Z, Brocklesby R, Luque R: Food waste as a valuable resource for the production of chemicals, materials and fuels. Current situation and global perspective. Energ Environ Sci 2013, 6:426-464.
  • [14]Uncu ON, Cekmecelioglu D: Cost-effective approach to ethanol production and optimization by response surface methodology. Waste Manage 2011, 31:636-643.
  • [15]Luque R, Clark J: Valorisation of food residues: waste to wealth using green chemical technologies. Sustain Chem Process 2013, 1:10. BioMed Central Full Text
  • [16]Arancon RAD, Lin CSK, Chan KM, Kwan TH, Luque R: Advances on waste valorization: new horizons for a more sustainable society. Energ Sci Eng 2013, 1:53-71.
  • [17]Jensen JW, Felby C, Jørgensen H, Rønsch GØ, Nørholm ND: Enzymatic processing of municipal solid waste. Waste Manage 2010, 30:2497-2503.
  • [18]Ma J, Duong TH, Smits M, Verstraete W, Carballa M: Enhanced biomethanation of kitchen waste by different pre-treatments. Bioresour Technol 2011, 102:592-599.
  • [19]Singhal S, Bansal SK, Singh R: Evaluation of biogas production from solid waste using pretreatment method in anaerobic condition. Int J Emerg Sci 2012, 2:405-414.
  • [20]Vavouraki AI, Angelis EM, Kornaros M: Optimization of thermo-chemical hydrolysis of kitchen wastes. Waste Manage 2013, 33:740-745.
  • [21]Singhania RR, Patel AK, Soccol CR, Pandey A: Recent advances in solid-state fermentation. Biochem Eng J 2009, 44:13-18.
  • [22]Jørgensen H, Vibe-Pedersen J, Larsen J, Felby C: Liquefaction of lignocellulose at high-solids concentrations. Biotechnol Bioeng 2007, 96:862-870.
  • [23]Larsen J, Østergaard Petersen M, Thirup L, Wen Li H, Krogh Iversen F: The IBUS process – lignocellulosic bioethanol close to a commercial reality. Chem Eng Technol 2008, 31:765-772.
  • [24]Bernstad A, Malmquist L, Truedsson C, la Cour Jansen J: Need for improvements in physical pretreatment of source-separated household food waste. Waste Manage 2013, 33:746-754.
  • [25]Kim JH, Lee JC, Pak D: Feasibility of producing ethanol from food waste. Waste Manage 2011, 31:2121-2125.
  • [26]Le Man H, Behera SK, Park HS: Optimization of operational parameters for ethanol production from Korean food waste leachate. Int J Environ Sci Tech 2010, 7:157-164.
  • [27]Zhang X, Richard T: Dual enzymatic saccharification of food waste for ethanol fermentation. Proceedings of international conference on electrical and control engineering: 16–18 September 2011; YichangISBN 978-1-4244-8162-0
  • [28]Szijarto N, Horan E, Zhang J, Puranen T, Siika-aho M, Viikari L: Thermostable endoglucanases in the liquefaction of hydrothermally pretreated wheat straw. Biotechnol Biofuels 2011, 4:2. BioMed Central Full Text
  • [29]Manzanares P, Negro MJ, Oliva JM, Saéz F, Ballesteros I, Ballesteros M, Cara C, Castro E, Ruiz E: Different process configurations for bioethanol production from pretreated olive pruning biomass. J Chem Technol Biotechnol 2011, 86:881-887.
  • [30]Hoyer K, Galbe M, Zacchi G: Production of fuel ethanol from softwood by simultaneous saccharification and fermentation at high dry matter content. J Chem Technol Biotechnol 2009, 84:570-577.
  • [31]Walker K, Vadlani P, Madl R, Ugorowski P, Hohn KL: Ethanol fermentation from food processing waste. Environ Prog Sustain Energ 2012, 32:1280-1283.
  • [32]Jeong S-M, Kim Y-J, Lee D-H: Ethanol production by co-fermentation of hexose and pentose from food wastes using Saccharomyces coreanus and Pichia stipitis. Korean J Chem Eng 2012, 29:1038-1043.
  • [33]Cekmecelioglu D, Uncu ON: Kinetic modeling of enzymatic hydrolysis of pretreated kitchen wastes for enhancing bioethanol production. Waste Manage 2013, 33:735-739.
  • [34]Kim JK, Oh BR, Shin H-J, Eom C-Y, Kim SW: Statistical optimization of enzymatic saccharification and ethanol fermentation using food waste. Process Biochem 2008, 43:1308-1312.
  • [35]Tang Y-Q, Koike Y, Liu K, An M-Z, Morimura S, Wu X-L, Kida K: Ethanol production from kitchen waste using the flocculating yeast Saccharomyces cerevisiae strain KF-7. Biomass Bioenerg 2008, 32:1037-1045.
  • [36]Dererie DY, Trobro S, Momeni MH, Hansson H, Blomqvist J, Passoth V, Schnürer A, Sandgren M, Ståhlberg J: Improved bio-energy yields via sequential ethanol fermentation and biogas digestion of steam exploded oat straw. Bioresour Technol 2011, 102:4449-4455.
  • [37]Bauer A, Bösch P, Friedl A, Amon T: Analysis of methane potentials of steam-exploded wheat straw and estimation of energy yields of combined ethanol and methane production. J Biotechnol 2009, 142:50-55.
  • [38]Kaparaju P, Serrano M, Thomsen AB, Kongjan P, Angelidaki I: Bioethanol, biohydrogen and biogas production from wheat straw in a biorefinery concept. Bioresour Technol 2009, 100:2562-2568.
  • [39]Bondesson PM, Galbe G, Zacchi G: Ethanol and biogas production after steam pretreatment of corn stover with or without the addition of sulphuric acid. Biotechnol Biofuels 2013, 6:11. BioMed Central Full Text
  • [40]Xiros C, Katapodis P, Christakopoulos P: Evaluation of Fusarium oxysporum cellulolytic system for an efficient hydrolysis of hydrothermally treated wheat straw. Bioresour Technol 2009, 100:5362-5365.
  • [41]Matsakas L, Christakopoulos P: Fermentation of liquefacted hydrothermally pretreated sweet sorghum bagasse to ethanol at high-solids content. Bioresour Technol 2013, 127:202-208.
  • [42]Petrik S, Kádár Z, Márová I: Utilization of hydrothermally pretreated wheat straw for production of bioethanol and carotene-enriched biomass. Bioresour Technol 2013, 133:370-377.
  • [43]da Cunha-Pereira F, Hickert LR, Sehnem NT, de Souza-Cruz PB, Rosa CA, Ayub MAZ: Conversion of sugars present in rice hull hydrolysates into ethanol by Spathaspora arborariae, Saccharomyces cerevisiae, and their co-fermentations. Bioresour Technol 2011, 102:4218-4225.
  • [44]Alvira P, Moreno AD, Ibarra D, Sáez F, Ballesteros M: Improving the fermentation performance of Saccharomyces cerevisiae by laccase during ethanol production from steam-exploded wheat straw at high-substrate loadings. Biotechnol Prog 2013, 29:74-82.
  • [45]Geddes CC, Peterson JJ, Roslander C, Zacchi G, Mullinnix MT, Shanmugam KT, Ingram LO: Optimizing the saccharification of sugar cane bagasse using dilute phosphoric acid followed by fungal cellulases. Bioresour Technol 2010, 101:1851-1857.
  • [46]Palmqvist E, Hahn-Hägerdal B: Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition. Bioresour Technol 2000, 74:25-33.
  • [47]Klinke HB, Thomsen AB, Ahring BK: Inhibition of ethanol-producing yeast and bacteria by degradation products produced during pre-treatment of biomass. Appl Microbiol Biotechnol 2004, 66:10-26.
  • [48]LIFE 08/ENV/GR/000566 [http://www.uest.gr/drywaste/site/index.htm webcite]
  • [49]Ghose TK: Measurement of cellulase activities. Pure Appl Chem 1987, 59:257-268.
  • [50]Miller GL: Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 1959, 31:426-428.
  • [51]Sluiter A, Ruiz R, Scarlata C, Sluiter J, Templeton D: Determination of Extractives in Biomass. Technical report NREL/TP-510-42619, Laboratory analytical protocol. Golden CO: National Renewable Energy Laboratory; 2008.
  • [52]Bradford MM: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72:248-254.
  • [53]Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D: Determination of structural carbohydrates and lignin biomass. Technical report NREL/TP-510-42618, Laboratory analytical protocol. Golden CO: National Renewable Energy Laboratory; 2012.
  • [54]William H: Official methods of analysis of the association of official analytical chemists. Washigton DC: AOAC Inc; 1970.
  • [55]Phatak L, Chang KC, Brown G: Isolation and characterization of pectin in sugar-beet pulp. J Food Sci 1988, 53:830-833.
  • [56]Karnaouri AC, Topakas E, Christakopoulos P: Cloning, expression, and characterization of a thermostable GH7 endoglucanase from Myceliophthora thermophila capable of high-consistency enzymatic liquefaction. Appl Microbiol Biotechnol 2013. in press
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