Phylogenetic groups and cephalosporin resistance genes of Escherichia coli from diseased food-producing animals in Japan

Acta Veterinaria Scandinavica

Tetsuo Asai²  Kaori Masani¹  Chizuru Sato¹  Mototaka Hiki²  Masaru Usui²  Kotaro Baba²  Manao Ozawa²  Kazuki Harada¹  Hiroshi Aoki¹  Takuo Sawada¹ 

[1] Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8601, Japan

[2] National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511

关键词: phylogenetic grouping;    AmpC β-lactamase;    extended spectrum β-lactamase;    cephalosporin resistance;    food-producing animals;    Escherichia coli;   

Others  :  789515
DOI  :  10.1186/1751-0147-53-52

 received in 2011-07-21, accepted in 2011-10-12,  发布年份 2011

【 摘 要 】

A total of 318 Escherichia coli isolates obtained from different food-producing animals affected with colibacillosis between 2001 and 2006 were subjected to phylogenetic analysis: 72 bovine isolates, 89 poultry isolates and 157 porcine isolates. Overall, the phylogenetic group A was predominant in isolates from cattle (36/72, 50%) and pigs (101/157, 64.3%) whereas groups A (44/89, 49.4%) and D (40/89, 44.9%) were predominant in isolates from poultry. In addition, group B2 was not found among diseased food-producing animals except for a poultry isolate. Thus, the phylogenetic group distribution of E. coli from diseased animals was different by animal species. Among the 318 isolates, cefazolin resistance (minimum inhibitory concentrations: ≥32 μg/ml) was found in six bovine isolates, 29 poultry isolates and three porcine isolates. Of them, 11 isolates (nine from poultry and two from cattle) produced extended spectrum β-lactamase (ESBL). The two bovine isolates produced bla_CTX-M-2, while the nine poultry isolates produced bla_CTX-M-25 (4), bla_SHV-2 (3), bla_CTX-M-15 (1) and bla_CTX-M-2 (1). Thus, our results showed that several types of ESBL were identified and three types of β-lactamase (SHV-2, CTX-M-25 and CTX-M-15) were observed for the first time in E. coli from diseased animals in Japan.

【 授权许可】

   
2011 Asai et al; licensee BioMed Central Ltd.

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