Microalgal lipid production using the hydrolysates of rice straw pretreated with gamma irradiation and alkali solution

Biotechnology for Biofuels

Min-Ho Joe¹  Ji-Youn Kim⁴  Sangyong Lim⁴  Dong-Ho Kim⁴  Suk Bai³  Hyun Park²  Sung Gu Lee²  Se Jong Han²  Jong-il Choi⁵ 

[1] School of Biological Sciences and Biotechnology, Chonnam National University, Gwangju 500-757, Republic of Korea

[2] Korea Polar Research Institute, Incheon 406-840, Republic of Korea

[3] Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Gwangju, Republic of Korea

[4] Department of Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 580-185, Republic of Korea

[5] Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 500-757, Republic of Korea

关键词: Lipid;    Gamma ray irradiation;    Microalgae;    Pretreatment;    Rice straw;   

Others  :  1228155
DOI  :  10.1186/s13068-015-0308-x

 received in 2015-05-08, accepted in 2015-08-07,  发布年份 2015

【 摘 要 】

Background

Lignocellulosic biomass has long been recognized as a potential sustainable source of sugars for biofuels. However, many physicochemical structural and compositional factors inhibit the enzymatic digestibility of the lignocellulosic biomass. In this study, efficient pretreatment method of rice straw (RS) was developed and the RS hydrolysate was applied in the cultivation of microalgae for lipid production.

Results

Gamma ray irradiation (GRI) and alkali solution were used for the pretreatment, and saccharification was carried out with lignocellulolytic enzymes. When RS was pretreated by combined GRI and alkali method, the glucose and xylose saccharification yield after enzymatic hydrolysis increased up to 91.65 and 98.84 %, respectively. The enzymatic hydrolysate from the RS pretreated with the combined method was used to cultivate Chlorella protothecoides for lipid production. The maximum concentrations of biomass and fatty acid methyl ester of cells were 6.51 and 2.95 g/L, respectively. The lipid content of C. protothecoides from RS hydrolysate was comparable to that from glucose, and the lipid composition was similar between different carbon sources.

Conclusion

These results demonstrate that the combined pretreatment with gamma irradiation was highly effective in preparing hydrolysate, and the rice straw hydrolysate could be used as an alternative carbon source for microalgal lipid production for biofuel.

【 授权许可】

   
2015 Joe et al.

【 图 表 】

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