Effects of lignin modification on wheat straw cell wall deconstruction by Phanerochaete chrysosporium

Biotechnology for Biofuels

Jijiao Zeng¹  Deepak Singh¹  Difeng Gao¹  Shulin Chen¹ 

[1] Department of Biological Systems Engineering, Bioprocessing and Bioproduct Engineering Laboratory (BBEL), Washington State University, L.J. Smith 213, Pullman 99163, Washington, USA

关键词: Cellulase enzyme lignin;    Milled wood lignin;    Inter-unit linkages;    Solid-state NMR;    Cellulase absorption;    Lignin-carbohydrate complex;    White rot fungi;    Lignin degradation;    NMR;   

Others  :  1084239
DOI  :  10.1186/s13068-014-0161-3

 received in 2014-05-10, accepted in 2014-10-16,  发布年份 2014

【 摘 要 】

Background

A key focus in sustainable biofuel research is to develop cost-effective and energy-saving approaches to increase saccharification of lignocellulosic biomass. Numerous efforts have been made to identify critical issues in cellulose hydrolysis. Aerobic fungal species are an integral part of the carbon cycle, equip the hydrolytic enzyme consortium, and provide a gateway for understanding the systematic degradation of lignin, hemicelluloses, and cellulose. This study attempts to reveal the complex biological degradation process of lignocellulosic biomass by Phanerochaete chrysosporium in order to provide new knowledge for the development of energy-efficient biorefineries.

Results

In this study, we evaluated the performance of a fungal biodegradation model, Phanerochaete chrysosporium, in wheat straw through comprehensive analysis. We isolated milled straw lignin and cellulase enzyme-treated lignin from fungal-spent wheat straw to determine structural integrity and cellulase absorption isotherms. The results indicated that P. chrysosporium increased the total lignin content in residual biomass and also increased the cellulase adsorption kinetics in the resulting lignin. The binding strength increased from 117.4 mL/g to 208.7 mL/g in milled wood lignin and from 65.3 mL/g to 102.4 mL/g in cellulase enzyme lignin. A detailed structural dissection showed a reduction in the syringyl lignin/guaiacyl lignin ratio and the hydroxycinnamate/lignin ratio as predominant changes in fungi-spent lignin by heteronuclear single quantum coherence spectroscopy.

Conclusion

P. chrysosporium shows a preference for degradation of phenolic terminals without significantly destroying other lignin components to unzip carbohydrate polymers. This is an important step in fungal growth on wheat straw. The phenolics presumably locate at the terminal region of the lignin moiety and/or link with hemicellulose to form the lignin-carbohydrate complex. Findings may inform the development of a biomass hydrolytic enzyme combination to enhance lignocellulosic biomass hydrolysis and modify the targets in plant cell walls.

【 授权许可】

   
2014 Zeng et al.; licensee BioMed Central Ltd.

【 图 表 】

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