Cellobionic acid utilization: from Neurospora crassa to Saccharomyces cerevisiae

Biotechnology for Biofuels

Xin Li²  Kulika Chomvong⁴  Vivian Yaci Yu²  Julie M Liang³  Yuping Lin²  Jamie H D Cate¹ 

[1] Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA

[2] Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA

[3] Department of Chemistry, University of California, Berkeley, CA, USA

[4] Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA

关键词: Cellobionic acid;    Biofuels;    Metabolic engineering;    Transporter;    Phosphorylase;    β-glucosidase;    AA9;    LPMO;    Aldonic acid;   

Others  :  1228585
DOI  :  10.1186/s13068-015-0303-2

 received in 2015-06-16, accepted in 2015-07-31,  发布年份 2015

【 摘 要 】

Background

Economical production of fuels and chemicals from plant biomass requires the efficient use of sugars derived from the plant cell wall. Neurospora crassa, a model lignocellulosic degrading fungus, is capable of breaking down the complex structure of the plant cell wall. In addition to cellulases and hemicellulases, N. crassa secretes lytic polysaccharide monooxygenases (LPMOs), which cleave cellulose by generating oxidized sugars—particularly aldonic acids. However, the strategies N. crassa employs to utilize these sugars are unknown.

Results

We identified an aldonic acid utilization pathway in N. crassa, comprised of an extracellular hydrolase (NCU08755), cellobionic acid transporter (CBT-1, NCU05853) and cellobionic acid phosphorylase (CAP, NCU09425). Extracellular cellobionic acid could be imported directly by CBT-1 or cleaved to gluconic acid and glucose by a β-glucosidase (NCU08755) outside the cells. Intracellular cellobionic acid was further cleaved to glucose 1-phosphate and gluconic acid by CAP. However, it remains unclear how N. crassa utilizes extracellular gluconic acid. The aldonic acid pathway was successfully implemented in Saccharomyces cerevisiae when N. crassa gluconokinase was co-expressed, resulting in cellobionic acid consumption in both aerobic and anaerobic conditions.

Conclusions

We successfully identified a branched aldonic acid utilization pathway in N. crassa and transferred its essential components into S. cerevisiae, a robust industrial microorganism.

【 授权许可】

   
2015 Li et al.

【 图 表 】

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