Biotechnology for Biofuels | |
Enzyme affinity to cell types in wheat straw (Triticum aestivum L.) before and after hydrothermal pretreatment | |
Mads AT Hansen3  Budi J Hidayat3  Kit K Mogensen4  Martin D Jeppesen4  Bodil Jørgensen2  Katja S Johansen1  Lisbeth G Thygesen3  | |
[1] Novozymes A/S, Krogshøjvej 36, Bagsværd, Denmark | |
[2] Department of Agriculture and Ecology/Department of Plant and Environment Sciences, University of Copenhagen, Thorvaldsensvej 40, Frederiksberg C DK-1871, Denmark | |
[3] Forest and Landscape, University of Copenhagen, Rolighedsvej 23, Frederiksberg C DK-1958, Denmark | |
[4] Inbicon A/S, Kraftværksvej 5, Skærbæk, Fredericia DK-7000, Denmark | |
关键词: Biomass characterisation; Enzymatic hydrolysis; Plant tissues; Wheat straw; Cellulosic biofuels; | |
Others : 798097 DOI : 10.1186/1754-6834-6-54 |
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received in 2012-11-18, accepted in 2013-04-10, 发布年份 2013 | |
【 摘 要 】
Background
Wheat straw used for bioethanol production varies in enzymatic digestibility according to chemical structure and composition of cell walls and tissues. In this work, the two biologically different wheat straw organs, leaves and stems, are described together with the effects of hydrothermal pretreatment on chemical composition, tissue structure, enzyme adhesion and digestion. To highlight the importance of inherent cell wall characteristics and the diverse effects of mechanical disruption and biochemical degradation, separate leaves and stems were pretreated on lab-scale and their tissue structures maintained mostly intact for image analysis. Finally, samples were enzymatically hydrolysed to correlate digestibility to chemical composition, removal of polymers, tissue composition and disruption, particle size and enzyme adhesion as a result of pretreatment and wax removal. For comparison, industrially pretreated wheat straw from Inbicon A/S was included in all the experiments.
Results
Within the same range of pretreatment severities, industrial pretreatment resulted in most hemicellulose and epicuticular wax/cutin removal compared to lab-scale pretreated leaves and stems but also in most re-deposition of lignin on the surface. Tissues were furthermore degraded from tissues into individual cells while lab-scale pretreated samples were structurally almost intact. In both raw leaves and stems, endoglucanase and exoglucanase adhered most to parenchyma cells; after pretreatment, to epidermal cells in all the samples. Despite heavy tissue disruption, industrially pretreated samples were not as susceptible to enzymatic digestion as lab-scale pretreated leaves while lab-scale pretreated stems were the least digestible.
Conclusions
Despite preferential enzyme adhesion to epidermal cells after hydrothermal pretreatment, our results suggest that the single most important factor determining wheat straw digestibility is the fraction of parenchyma cells rather than effective tissue disruption.
【 授权许可】
2013 Hansen et al.; licensee BioMed Central Ltd.
【 预 览 】
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