Comparing the effects of isoflurane and pentobarbital on the responses of cutaneous mechanoreceptive afferents

BMC Anesthesiology

Ju-Wen Cheng¹  Alison I Weber²  Sliman J Bensmaia² 

[1] Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan; Department of Organismal Biology and Anatomy, University of Chicago, Chicago, IL, USA

[2] Department of Organismal Biology and Anatomy, University of Chicago, Chicago, IL, USA

关键词: Entrainment;    Vibration;    Pentobarbital;    Isoflurane;    Anesthesia;    Mechanoreceptive afferents;   

Others  :  816862
DOI  :  10.1186/1471-2253-13-10

 received in 2012-07-25, accepted in 2013-04-16,  发布年份 2013

【 摘 要 】

Background

While pentobarbital has been used extensively in neurophysiological experiments investigating activity in peripheral nerves, it has fallen out of favor as an anesthetic because of safety concerns and is often replaced with isoflurane. However, the effects of isoflurane on the excitability of mechanoreceptive afferents have yet to be conclusively elucidated.

Methods

To fill this gap, we collected extracellular single-unit recordings of cutaneous mechanoreceptive afferents from the sciatic nerve of 21 rats during vibratory stimulation of the hindpaw. We then compared the strength and temporal structure of the afferent response measured under pentobarbital and isoflurane anesthesia.

Results

We found that the strength and temporal structure of afferent responses were statistically equivalent whether these were evoked under isoflurane or pentobarbital.

Conclusions

We conclude that, if these two anesthetics have any effect on the responses of mechanoreceptive afferents, their effects are indistinguishable.

【 授权许可】

   
2013 Cheng et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

Figure 6.

【 参考文献 】

• [1]Stokes EL, Flecknell PA, Richardson CA: Reported analgesic and anaesthetic administration to rodents undergoing experimental surgical procedures. Lab Anim 2009, 43(2):149-154.
• [2]Feldman S, Wagman IH: The Effect of Pentobarbital on Evoked Potentials in Brain of Macaca Mulatta. Electroencephalogr Clin Neurophysiol 1963, 15:747-760.
• [3]Collins JG, Roppolo JR: Effects of pentobarbital and ethanol upon single-neuron activity in the primary somatosensory cortex of the rhesus monkey. J Pharmacol Exp Ther 1980, 213(2):337-345.
• [4]Calancie B, Klose KJ, Baier S, Green BA: Isoflurane-induced attenuation of motor evoked potentials caused by electrical motor cortex stimulation during surgery. J Neurosurg 1991, 74(6):897-904.
• [5]Mason DG, Higgins D, Boyd SG, Lloyd-Thomas AR: Effects of isoflurane anaesthesia on the median nerve somatosensory evoked potential in children. Br J Anaesth 1992, 69(6):562-566.
• [6]Samra SK, Vanderzant CW, Domer PA, Sackellares JC: Differential effects of isoflurane on human median nerve somatosensory evoked potentials. Anesthesiology 1987, 66(1):29-35.
• [7]Cheung SW, Nagarajan SS, Bedenbaugh PH, Schreiner CE, Wang X, Wong A: Auditory cortical neuron response differences under isoflurane versus pentobarbital anesthesia. Hear Res 2001, 156(1–2):115-127.
• [8]Tomsic M, Bajrovic F: Sensitivity of peripheral nerve fibres to sodium pentobarbital anaesthesia in rat. Pflugers Archiv : European journal of physiology 2000, 440(5 Suppl):R107-108.
• [9]MacIver MB, Tanelian DL: Volatile anesthetics excite mammalian nociceptor afferents recorded in vitro. Anesthesiology 1990, 72(6):1022-1030.
• [10]Vahle-Hinz C, Detsch O: What can in vivo electrophysiology in animal models tell us about mechanisms of anaesthesia? Br J Anaesth 2002, 89(1):123-142.
• [11]Oh SS, Hayes JM, Sims-Robinson C, Sullivan KA, Feldman EL: The effects of anesthesia on measures of nerve conduction velocity in male C57Bl6/J mice. Neurosci Lett 2010, 483(2):127-131.
• [12]Puil E, Gimbarzevsky B: Modifications in membrane properties of trigeminal sensory neurons during general anesthesia. J Neurophysiol 1987, 58(1):87-104.
• [13]Antognini JF, Carstens E, Tabo E, Buzin V: Effect of differential delivery of isoflurane to head and torso on lumbar dorsal horn activity. Anesthesiology 1998, 88(4):1055-1061.
• [14]Devecioglu I, Guclu B: Asymmetric response properties of rapidly adapting mechanoreceptive fibers in the rat glabrous skin. Somatosensory & motor research 2012.
• [15]Talbot WH, Darian-Smith I, Kornhuber HH, Mountcastle VB: The sense of flutter-vibration: comparison of the human capacity with response patterns of mechanoreceptive afferents from the monkey hand. Journal of neurophysiology 1968, 31(2):301-334.
• [16]Fox JG, Anderson LC, Loew FM, Quimby FW (Eds): Laboratory Animal Medicine. London, UK: Academic Press; http://site.ebrary.com/id/10206370 webcite
• [17]Grün S, Rotter S: Analysis of Parallel Spike Trains. Springer; 2010:59-67.
• [18]Sahaghian R, Faith NG, Czuprynski C: Comparison of systemic Listeria monocytogenes infection in esophageally inoculated mice anesthetized with isoflurane or pentobarbital. Lab animal 2009, 38(4):126-130.
• [19]Hunt SP, Koltzenburg M: The neurobiology of pain: (molecular and cellular neurobiology). Oxford University Press; 2005:2-3.
• [20]Chen M, Olsen JI, Stolk JA, Schweizer MP, Sha M, Ueda I: An in vivo 19F NMR study of isoflurane elimination as a function of age in rat brain. NMR Biomed 1992, 5(3):121-126.
• [21]Kameyama K, Aono K, Kitamura K: Isoflurane inhibits neuronal Ca2+ channels through enhancement of current inactivation. Br J Anaesth 1999, 82(3):402-411.
• [22]Antognini JF, Kien ND: Potency (minimum alveolar anesthetic concentration) of isoflurane is independent of peripheral anesthetic effects. Anesth Analg 1995, 81(1):69-72.
• [23]Kitahara S, Yamashita M, Ikemoto Y: Effects of pentobarbital on purinergic P2X receptors of rat dorsal root ganglion neurons. Can J Physiol Pharmacol 2003, 81(12):1085-1091.
• [24]Valeyev AY, Hackman JC, Holohean AM, Wood PM, Davidoff RA: Pentobarbital-activated Cl(−) channels in cultured adult and embryonic human DRG neurons. Brain Res Dev Brain Res 2000, 124(1–2):137-140.
• [25]Mountcastle VB: The sensory hand: neural mechanisms of somatic sensation. Harvard University Press; 2005.
• [26]Phillips JR, Johansson RS, Johnson KO: Responses of human mechanoreceptive afferents to embossed dot arrays scanned across fingerpad skin. The Journal of neuroscience : the official journal of the Society for Neuroscience 1992, 12(3):827-839.