期刊论文详细信息
BioMedical Engineering OnLine
Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy
Rong Dong1  Jinhua Long5  Xiaoli Xu1  Chunlin Zhang1  Zongyao Wen3  Long Li2  Weijuan Yao3  Zhu Zeng4 
[1] Department of Cell Biology, Guiyang Medical College, Guiyang, Guizhou Province 550004, People’s Republic of China
[2] Department of Nephrology, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou Province 550004, P. R. China
[3] Center of Hemorheology, School of Basic Medical Sciences, Health Science Center of Peking University, Beijing 100083, People’s Republic of China
[4] Department of Biotechnology, Guiyang Medical College, Guiyang, Guizhou Province 550004, P. R. China
[5] Department of Head and Neck, Affiliated Cancer Hospital, Guiyang Medical College, Guiyang, Guizhou Province 550004, People’s Republic of China
关键词: NF-κB;    Functional states of cells;    Fourier transformed infrared spectroscopy;    Dendritic cell;   
Others  :  797227
DOI  :  10.1186/1475-925X-13-2
 received in 2013-11-02, accepted in 2014-01-04,  发布年份 2014
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【 摘 要 】

Background

Dendritic cells are potent and specialized antigen presenting cells, which play a crucial role in initiating and amplifying both the innate and adaptive immune responses. The dendritic cell-based vaccination against cancer has been clinically achieved promising successes. But there are still many challenges in its clinical application, especially for how to identify the functional states.

Methods

The CD14+ monocytes were isolated from human peripheral blood after plastic adherence and purified to approximately 98% with cocktail immunomagnetic beads. The immature dendritic cells and mature dendritic cells were induced by traditional protocols. The resulting dendritic cells were cocultured with normal cells and cancer cells. The functional state of dendritic cells including immature dendritic cells (imDCs) and mature dendritic cells (mDCs) under different conditioned microenvironments were investigated by Fourier transformed infrared spectroscopy (FTIR) and molecular biological methods.

Results

The results of Fourier transformed infrared spectroscopy showed that the gene transcription activity and energy states of dendritic cells were specifically suppressed by tumor cells (P < 0.05 or 0.01). The expression levels of NF-kappa B (NF-κB) in dendritic cells were also specifically inhibited by tumor-derived factors (P < 0.05 or 0.01). Moreover, the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were closely correlated with the expression levels of NF-κB (R2:0.69 and R2:0.81, respectively).

Conclusion

Our results confirmed that the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were positively correlated with the expression levels of NF-κB, suggesting that Fourier transformed infrared spectroscopy technology could be clinically applied to identify the functional states of dendritic cell when performing dendritic cell-based vaccination. It’s significant for the simplification and standardization of dendritic cell-based vaccination clinical preparation protocols.

【 授权许可】

   
2014 Dong et al.; licensee BioMed Central Ltd.

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