Histopathological findings, phenotyping of inflammatory cells, and expression of markers of nitritative injury in joint tissue samples from calves after vaccination and intraarticular challenge with Mycoplasma bovis strain 1067

Acta Veterinaria Scandinavica

Vemuri Rama Devi¹  François Poumarat³  Dominique Le Grand⁵  Renate Rosengarten²  Kathrin Hermeyer⁴  Marion Hewicker-Trautwein⁶ 

[1] Current address: Department of Veterinary Pathology, NTR College of Veterinary Science, Sri Venkateswara Veterinary University, Gannavaram 521 102, India

[2] Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine Vienna, Veterinärplatz 1, Vienna, A-1210, Austria

[3] Anses, Lyon Laboratory, UMR Mycoplasmoses des Ruminants, 31 Avenue Tony Garnier, Lyon, F-69364 cedex 07, France

[4] Current address: Vet Med Labor GmbH, Division of IDEXX Laboratories, Mörikestrasse 28/3, Ludwigsburg, D-71636, Germany

[5] Université de Lyon, VetAgro Sup, UMR Mycoplasmoses des Ruminants, 1 Avenue Bourgelat, Marcy-L’Étoile, F-69280, France

[6] Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, Hannover, D-30559, Germany

关键词: Nitrotyrosine;    Inducible nitric oxide;    MHC class II;    Variable surface protein antigens;    Vaccination;    Arthritis;    Mycoplasma bovis;   

Others  :  1082568
DOI  :  10.1186/s13028-014-0045-3

 received in 2014-02-06, accepted in 2014-06-30,  发布年份 2014

【 摘 要 】

Background

The pathogenesis of caseonecrotic lesions developing in lungs and joints of calves infected with Mycoplasma bovis is not clear and attempts to prevent M. bovis-induced disease by vaccines have been largely unsuccessful. In this investigation, joint samples from 4 calves, i.e. 2 vaccinated and 2 non-vaccinated, of a vaccination experiment with intraarticular challenge were examined. The aim was to characterize the histopathological findings, the phenotypes of inflammatory cells, the expression of class II major histocompatibility complex (MHC class II) molecules, and the expression of markers for nitritative stress, i.e. inducible nitric oxide synthase (iNOS) and nitrotyrosine (NT), in synovial membrane samples from these calves. Furthermore, the samples were examined for M. bovis antigens including variable surface protein (Vsp) antigens and M. bovis organisms by cultivation techniques.

Results

The inoculated joints of all 4 calves had caseonecrotic and inflammatory lesions. Necrotic foci were demarcated by phagocytic cells, i.e. macrophages and neutrophilic granulocytes, and by T and B lymphocytes. The presence of M. bovis antigens in necrotic tissue lesions was associated with expression of iNOS and NT by macrophages. Only single macrophages demarcating the necrotic foci were positive for MHC class II. Microbiological results revealed that M. bovis had spread to approximately 27% of the non-inoculated joints. Differences in extent or severity between the lesions in samples from vaccinated and non-vaccinated animals were not seen.

Conclusions

The results suggest that nitritative injury, as in pneumonic lung tissue of M. bovis-infected calves, is involved in the development of caseonecrotic joint lesions. Only single macrophages were positive for MHC class II indicating down-regulation of antigen-presenting mechanisms possibly caused by local production of iNOS and NO by infiltrating macrophages.

【 授权许可】

   
2014 Devi et al.; licensee BioMed Central Ltd.

【 图 表 】

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