Localization of polyhydroxybutyrate in sugarcane using Fourier-transform infrared microspectroscopy and multivariate imaging

Biotechnology for Biofuels

Robert J. Henry³  Blake A. Simmons²  Henrik V. Scheller⁴  Richard McQualter⁵  Seema Singh²  Andreia Smith-Moritz⁴  Jason S. Lupoi¹ 

[1]Sage Analytics, Boulder 80301, CO, USA

[2]Biological and Engineering Sciences Center, Sandia National Laboratories, 7011 East Avenue, Livermore 94551, CA, USA

[3]Queensland Alliance for Agriculture and Food Innovation, University of Queensland, St. Lucia 4072, Queensland, Australia

[4]Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, 5885 Hollis Street, Emeryville 94608, CA, USA

[5]Department of Biological Sciences, University of North Texas, 1155 Union Circle #305220, Denton 76203, TX, USA

关键词: Multivariate imaging;    Sugarcane;    Polyhydroxybutyrate;    Focal plane array;    Infrared imaging;   

Others  :  1221329
DOI  :  10.1186/s13068-015-0279-y

 received in 2015-02-16, accepted in 2015-06-26,  发布年份 2015

【 摘 要 】

Background

Slow-degrading, fossil fuel-derived plastics can have deleterious effects on the environment, especially marine ecosystems. The production of bio-based, biodegradable plastics from or in plants can assist in supplanting those manufactured using fossil fuels. Polyhydroxybutyrate (PHB) is one such biodegradable polyester that has been evaluated as a possible candidate for relinquishing the use of environmentally harmful plastics.

Results

PHB, possessing similar properties to polyesters produced from non-renewable sources, has been previously engineered in sugarcane, thereby creating a high-value co-product in addition to the high biomass yield. This manuscript illustrates the coupling of a Fourier-transform infrared microspectrometer, equipped with a focal plane array (FPA) detector, with multivariate imaging to successfully identify and localize PHB aggregates. Principal component analysis imaging facilitated the mining of the abundant quantity of spectral data acquired using the FPA for distinct PHB vibrational modes. PHB was measured in the chloroplasts of mesophyll and bundle sheath cells, acquiescent with previously evaluated plant samples.

Conclusion

This study demonstrates the power of IR microspectroscopy to rapidly image plant sections to provide a snapshot of the chemical composition of the cell. While PHB was localized in sugarcane, this method is readily transferable to other value-added co-products in different plants.

【 授权许可】

   
2015 Lupoi et al.

【 图 表 】

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