Self-sampling is appropriate for detection of Staphylococcus aureus: a validation study

Antimicrobial Resistance and Infection Control

Brigitte AGL van Cleef¹  Miranda van Rijen¹  Marianne Ferket¹  Jan AJW Kluytmans² 

[1] Amphia Hospital Breda, Laboratory for Microbiology and Infection Control, Breda, the Netherlands

[2] VU University medical centre, Department of Medical Microbiology and Infection Prevention, Amsterdam, the Netherlands

关键词: Validation;    MRSA;    Staphylcococcus aureus;    Self-sampling;   

Others  :  791081
DOI  :  10.1186/2047-2994-1-34

 received in 2012-09-17, accepted in 2012-11-06,  发布年份 2012

【 摘 要 】

Background

Studies frequently use nasal swabs to determine Staphylococcus aureus carriage. Self-sampling would be extremely useful in an outhospital research situation, but has not been studied in a healthy population. We studied the similarity of self-samples and investigator-samples in nares and pharynxes of healthy study subjects (hospital staff) in the Netherlands.

Methods

One hundred and five nursing personnel members were sampled 4 times in random order after viewing an instruction paper: 1) nasal self-sample, 2) pharyngeal self-sample, 3) nasal investigator-sample, and 4) pharyngeal investigator-sample.

Results

For nasal samples, agreement is 93% with a kappa coefficient of 0.85 (95% CI 0.74-0.96), indicating excellent agreement, for pharyngeal samples agreement is 83% and the kappa coefficient is 0.60 (95% CI 0.43-0.76), indicating good agreement. In both sampling sites self-samples even detected more S. aureus than investigator-samples.

Conclusions

This means that self-samples are appropriate for detection of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus.

【 授权许可】

   
2012 van Cleef et al.; licensee BioMed Central Ltd.

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