Effect of Fe nanoparticle on growth and glycolipid biosurfactant production under solid state culture by marine Nocardiopsis sp. MSA13A

BMC Biotechnology

George Seghal Kiran¹  Lipton Anuj Nishanth²  Sethu Priyadharshini¹  Kumar Anitha¹  Joseph Selvin² 

[1] Department of Food Science and Technology, Pondicherry University, Puducherry 605014, India

[2] Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry 605014, India

关键词: Nanoparticle-microbial interaction;    Solid state fermentation;    Actinobacterium;    Biosurfactant;    Fe nanoparticles;   

Others  :  834840
DOI  :  10.1186/1472-6750-14-48

 received in 2013-10-15, accepted in 2014-04-17,  发布年份 2014

【 摘 要 】

Background

Iron is an essential element in several pathways of microbial metabolism, and therefore low iron toxicity is expected on the usage of Fe nanoparticles (NPs). This study aims to determine the effect of Fe NPs on biosurfactant production by marine actinobacterium Nocardiopsis sp. MSA13A under solid state culture. Foam method was used in the production of Fe NPs which were long and fiber shaped in nature.

Results

The SEM observation showed non toxic nature of Fe NPs as no change in the morphology of the filamentous structure of Nocardiopsis MSA13A. The production of biosurfactant by Nocardiopsis MSA13A under solid state culture supplemented with Fe NPs increased to 80% over control. The biosurfactant produced by Nocardiopsis MSA13A was characterized as glycolipid derivative which effectively disrupted the pre-formed biofilm of Vibrio pathogen.

Conclusion

The use of metal NPs as supplement would reduce the impact of non-metallic ions of the metal salts in a fermentation process. This would ultimately useful to achieve greener production process for biosurfactants. The present results are first report on the optimization of biosurfactant production under SSC using Fe NPs.

【 授权许可】

   
2014 Kiran et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

Figure 6.

【 参考文献 】

• [1]Cameotra SS, Makkar R: Synthesis of biosurfactants in extreme conditions. Appl Microbiol Biotechnol 1998, 50:520-529.
• [2]Khopade A, Biao R, Liu X, Mahadik K, Zhang L, Kokare C: Production and stability studies of the biosurfactant isolated from marine Nocardiopsis sp. B4. Desalination 2012, 285:198-204.
• [3]Kiran GS, Selvin J, Manilal A, Sujith S: Biosurfactants as green stabilizer for the biological synthesis of nanoparticles. Crit Rev Biotechnol 2011, 31:354-364.
• [4]Haferburg G, Kothe E: Microbes and metals: interactions in the environment. J Basic Microbiol 2007, 47:453-467.
• [5]Kiran GS, Sabu A, Selvin J: Synthesis of silver nanoparticles by glycolipid biosurfactant produced from marine Brevibacterium casei MSA19. J Biotechnol 2010, 148:221-225.
• [6]Kiran GS, Anto Thomas T, Selvin J: Production of a new glycolipid biosurfactant from marine Nocardiopsis lucentensis MSA04 in solid-state cultivation. Coll Surf B: Biointerf 2010, 78:8-16.
• [7]Makkar RS, Cameotra SS: An update on the use of unconventional substrates for biosurfactants production and their new applications. Appl Microbiol Biotechnol 2002, 58:428-434.
• [8]Flores M, Colon N, Rivera O, Villalba N, Baez Y, Quispitupa D, Avalos J, Perales O: A study of the growth curves of C. xerosis and E. coli bacteria in mediums containing cobalt ferrite nanoparticles. Mat Res Soc Symp Proc 2004, 820.
• [9]Merceda T, Santosa S, Riveraa O, Villalbaa N, Baeza Y, Gaudiera J, Avalosa J, Peralesa O, Tomara MS, Parra-Palominoa A, Avalos J: Effect of zinc oxide nanocrystals in media containing E. coli and C. xerosis bacteria. Mater Res Soc Symp Proc 2006., 900
• [10]Das SK, Das AR, Guha AK: Gold nanoparticles: microbial synthesis and application in water hygiene management. Langmuir 2009, 25:8192-8199.
• [11]Sjogren CE, Johansson C, Naevestad A, Sontum PC, Saebo BK, Fahlvik AK: Crystal size and properties of superparamagnetic iron oxide (SPIO) particles. Magn Reson Imaging 1997, 15:55-67.
• [12]Perez JM, O’Loughin T, Simeone FJ, Weissleder R, Josephson L: DNA-based magnetic nanoparticle assembly acts as a magnetic relaxation nanoswitch allowing screening of DNA cleaving agents. J Am Chem Soc 2002, 124:2856-2857.
• [13]Fayati MD, Rahe EK, Md Angazi AT, Alizeh SK, Barani HN: The effect of magnetic Fe3o4 nanoparticles on genetically manipulated bacterium, Pseudomonas aeruginosa (P SOX4). Iran J Biotech 2013, 11:41-46.
• [14]Liu J, Vipulanandan C, Cooper TF, Vipulanandan G: Effects of Fe nanoparticles on bacterial growth and biosurfactant production. J Nanopart Res 2013, 15:1405.
• [15]Montalvo NF, Mohamed NM, Enticknap JJ, Hill RT: Novel actinobacteria from marine sponges. Antonie Van Leeuwenhoek 2005, 87:29-36.
• [16]Selvin J, Shanmughapriya S, Gandhimathi R, Seghal KG, Rajeetha Ravji T, Natarajaseenivasan K, Hema TA: Optimization and production of novel antimicrobial agents from sponge associated marine actinomycetes Nocardiopsis dassonvillei MAD08. Appl Microbiol Biotechnol 2009a, 83:435-445.
• [17]Meyer B, Kuever J: Phylogenetic diversity and spatial distribution of the microbial community associated with the caribbean deep-water sponge Polymastia cf. corticata by 16S rRNA, aprA, and amoA gene analysis. Microbial Ecol 2007, 56:306-321.
• [18]Blanco M, Meira A, Baldomir D, Rivas J, Lopez MA: UV–VIS spectra of small iron particles. IEEE Trans Mag 1994, 30:739-741.
• [19]Chae SR, Therezien M, Budarz JF, Wessel L, Lin S, Xiao Y, Wiesner MR: Comparison of the photosensitivity and bacterial toxicity of spherical and tubular fullerenes of variable aggregate size. J Nanopart Res 2011, 13:5121-5127.
• [20]Sadiq IM, Pakrashi S, Chandrasekaran N, Mukherjee A: Studies on toxicity of aluminum oxide (Al2O3) nanoparticles to microalgae species: Scenedesmus sp. and Chlorella sp. J Nanopart Res 2011, 13:3287-3299.
• [21]Choi O, Yu CP, Fernandez GE, Hu ZQ: Interactions of nanosilver with Escherichia coli cells in planktonic and biofilm cultures. Water Res 2010, 44:6095-6103.
• [22]Li F, Lei C, Shen Q, Li L, Wang M, Guo M, Huang Y, Nie Z, Yao S: Analysis of copper nanoparticles toxicity based on a stress-responsive bacterial biosensor array. Nanoscale 2013, 5:653-662.
• [23]Ehrlich HL: Microbes and metals. Appl Microbiol Biotechnol 1997, 48:687-692.
• [24]Hommel RK, Ratledge C: Biosynthetic mechanisms of low molecular weight surfactants and their precursor molecules. In Biosurfactant: Production, properties and applications. Edited by Kosaric N. Marcel Bekker Inc NY; 1993:3-63.
• [25]Bagg A, Neilands JB: Ferric uptake regulation protein acts as a repressor, employing iron II as a cofactor to bind the operator of an iron transporter operon in E.coliFerric uptake regulation protein acts as a repressor, employing iron II as a cofactor to bind the operator of an iron transporter operon in E.coli. Biochem 1987, 2617:5471-5477.
• [26]Petersson G: Gas chromatography–mass spectrometry of sugars and related hydroxy acids as trimethylsilyl derivatives. Svensk Papperstidning 1974, 77:5.
• [27]Selvin J, Lipton AP: Vibrio alginolyticus associated with white spot disease of Penaeus monodon. Dis Aquat Org 2003, 57:147-150.
• [28]He F, Zhao D, Liu J, Roberts CB: Stabilization of Fe-Pd nanoparticles with sodium carboxymethyl cellulose for enhanced transport and dechlorination of trichloroethylene in soil and groundwater. Ind Eng Chem Res 2006, 46:29-34.
• [29]He S, Feng Y, Ren H, Zhang Y, Gu N, Lin X: The impact of iron oxide magnetic nanoparticles on the soil bacterial community. J Soils Sediments 2011, 11:1408-1417.
• [30]Selvin J, Thangavelu T, Kiran GS, Gandhimathi R, Shanmughapriya S: Culturable heterotrophic bacteria from the marine sponge Dendrilla nigra: isolation and phylogenetic diversity of actinobacteria. Hel Mar Res 2009, 63:239-247.
• [31]Selvin J, Soniya J, Asha KRT, Manjusha WA, Sangeetha VS, Jayaseema DM, Antony MC, Vinitha DAJ: Antibacterial potential of antagonistic Streptomyces sp. isolated from the marine sponge Dendrilla nigra. FEMS Microbiol Ecol 2004, 50:117-122.
• [32]Morikawa M, Daido H, Takao T, Marato S, Shimonishi Y, Imanaka T: A new lipopeptide biosurfactant produced by Arthrobacter sp. strain MIS 38. J Bacteriol 1993, 175:6459-6466.
• [33]Kiran GS, Hema TA, Gandhimathi R, Selvin J, Manilal A, Sujith S, Natarajaseenivasan K: Optimization and production of a biosurfactant from the sponge-associated marine fungus Aspergillus ustus MSF3. Coll Surf B: Biointerf 2009, 73:250-256.
• [34]Paraszkiewicz K, Kanwal A, Dlugonski J: Emulsifier production by steroid transforming filamentous fungus Curvularia lunata, growth and product characterization. J Biotechnol 1992, 92:287-294.
• [35]Lechevalier MP: Ecological associations involving actinomycetes. In Actinomycetes. Edited by Schaal KP, Pulverer G. Stuttgart: Gustav Fischer Verlag; 1981:159-166.
• [36]Ferrara GB, Murgia B, Parodi AM, Valisano L, Cerrano C, Palmisano G, Bavestrello G, Sara M: The assessment of DNA from marine organisms via a modified salting-out protocol. Cell Mol Biol Lett 2006, 11:55-160.
• [37]Kiran GS, Thomas TA, Selvin J, Sabarathnam B, Lipton AP: Optimization and characterization of a new lipopeptide biosurfactant produced by marine Brevibacteriumaureum MSA13 in solid state culture. Bioresour Technol 2010, 101:2389-2396.
• [38]Montgomery DC: Analysis and design of experiments. 4th edition. New York, NY: Wiley; 1997.
• [39]Neto DC, Meira JA, de Araujo JM, Mitchell DA, Krieger N: Optimization of the production of rhamnolipids by Pseudomonas aeruginosa UFPEDA 614 in solid-state culture. Appl Microbiol Biotech 2008, 81:441-448.
• [40]Dubois M, Gilies K, Hammilton JK, Robers PA, Smith FA: A colorimetric method for the determinationof sugars related substances. Anal Chem 1951, 28:350-356.
• [41]Benincasa M: Rhamnolipid production by P. aeruginosa LBI growing on soap-stock as the sole carbon source. J Food Eng 2002, 3:283-288.