T-cell phenotypes in chronic rhinosinusitis with nasal polyps in Japanese patients

Allergy, Asthma & Clinical Immunology

Tatsuya Yamasoba²  Ken Ohta¹  Maho Suzukawa¹  Kenji Kondo²  Ryoji Kagoya²  Shintaro Baba³ 

[1]National Hospital Organization Tokyo National Hospital, Kiyose, Tokyo, Japan

[2]Department of Otolaryngology, Faculty of Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan

[3]Department of Otolaryngology, Tokyo Metropolitan Children’s Medical Center, Tokyo, Japan

关键词: Nasal polyps;    T cells;    Th1/Th2;    Th17;    Treg;   

Others  :  1234055
DOI  :  10.1186/s13223-015-0100-2

 received in 2015-08-09, accepted in 2015-10-19,  发布年份 2015

【 摘 要 】

Background

Chronic rhinosinusitis with nasal polyps is characterized by local inflammation and is categorized into two subtypes inJapan: eosinophilic chronic rhinosinusitis, and non-eosinophilic chronic rhinosinusitis. The objective of this study was to investigate the expression of key transcription factors for Treg and Th1/Th2/Th17 cells, in relation to the mRNA expression of representative cytokines in these two subtypes of chronic rhinosinusitis with nasal polyps.

Methods

The expression of forkhead box P3 (FOXP3), T-box transcription factor (T-bet), GATA3, retinoid acid-related orphan receptor C (RORc), the suppressive cytokines TGF-β1 and IL-10, and Th1/Th2/Th17 cytokines (IFN-γ, IL-4, IL-5, IL-13, IL-17) were analyzed by means of RT-PCR in eosinophilic polyps. Eosinophilic polyps were defined as having an eosinophil count of more than 50 per microscopic field (×400 magnification) using five fields located in the subepithelial area of the polyps, while the non-eosinophilic polyps and controls did not fulfill this criteria. The numbers of T cells, CD4+ T cells, CD8+ T cells and Treg were histologically counted using sections that were immunostained for CD3, CD4, CD8, and FOXP3, respectively.

Results

In eosinophilic polyps, we observed significantly fewer CD4+ T cells and CD8+ T cells, and lower GATA3, RORc and IL-10 mRNA expression, but a significantly higher IL-5, and IL-13 mRNA expression compared with controls, whereas FOXP3 and T-bet mRNA expression were not significantly different compared with controls. In non-eosinophilic polyps, FOXP3, IL-10, IL-17A, TGFβ1 and IFNγ mRNA expression was significantly higher compared with controls, whereas IL-4, 5 and 13 expression was not significantly different from controls.

Conclusion

We showed a reduction of GATA3 and RORc mRNA, low Treg-related cytokines and elevated Th2 cytokine levels in eosinophilic chronic rhinosinusitis, whereas we demonstrated the upregulation of Treg cells and increases of Th1 and Th17 cytokines in non-eosinophilic chronic rhinosinusitis in the Japanese population. The different mRNA expression profiles of Treg and Th1/Th2/Th17 signature transcription factors and cytokines between eosinophilic chronic rhinosinusitis and non-eosinophilic chronic rhinosinusitis suggests heterogeneity in the pathogenesis of chronic rhinosinusitis with nasal polyps.

【 授权许可】

   
2015 Baba et al.

【 图 表 】

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