Simultaneous hydrogen and ethanol production from cascade utilization of mono-substrate in integrated dark and photo-fermentative reactor

Biotechnology for Biofuels

Bing-Feng Liu¹  Guo-Jun Xie²  Rui-Qing Wang¹  De-Feng Xing¹  Jie Ding¹  Xu Zhou²  Hong-Yu Ren¹  Chao Ma¹  Nan-Qi Ren¹ 

[1] State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China

[2] Advanced Water Management Centre, The University of Queensland, St. Lucia QLD 4072, Australia

关键词: Membrane;    Kinetics;    Integrated dark and photo-fermentative reactor;    Photo-fermentation;    Dark-fermentation;    Ethanol production;    Hydrogen production;   

Others  :  1089691
DOI  :  10.1186/s13068-014-0191-x

 received in 2014-09-09, accepted in 2014-12-18,  发布年份 2015

【 摘 要 】

Background

Integrating hydrogen-producing bacteria with complementary capabilities, dark-fermentative bacteria (DFB) and photo-fermentative bacteria (PFB), is a promising way to completely recover bioenergy from waste biomass. However, the current coupled models always suffer from complicated pretreatment of the effluent from dark-fermentation or imbalance between dark and photo-fermentation, respectively. In this work, an integrated dark and photo-fermentative reactor (IDPFR) was developed to completely convert an organic substrate into bioenergy.

Results

In the IDPFR, Ethanoligenens harbinese B49 and Rhodopseudomonas faecalis RLD-53 were separated by a membrane into dark and photo chambers, while the acetate produced by E. harbinese B49 in the dark chamber could freely pass through the membrane into the photo chamber and serve as a carbon source for R. faecalis RLD-53. The hydrogen yield increased with increasing working volume of the photo chamber, and reached 3.38 mol H₂/mol glucose at the dark-to-photo chamber ratio of 1:4. Hydrogen production by the IDPFR was also significantly affected by phosphate buffer concentration, glucose concentration, and ratio of dark-photo bacteria. The maximum hydrogen yield (4.96 mol H₂/mol glucose) was obtained at a phosphate buffer concentration of 20 mmol/L, a glucose concentration of 8 g/L, and a ratio of dark to photo bacteria of 1:20. As the glucose and acetate were used up by E. harbinese B49 and R. faecalis RLD-53, ethanol produced by E. harbinese B49 was the sole end-product in the effluent from the IDPFR, and the ethanol concentration was 36.53 mmol/L with an ethanol yield of 0.82 mol ethanol/mol glucose.

Conclusions

The results indicated that the IDPFR not only circumvented complex pretreatments on the effluent in the two-stage process, but also overcame the imbalance of growth and metabolic rate between DFB and PFB in the co-culture process, and effectively enhanced cooperation between E. harbinense B49 and R. faecalis RLD-53. Moreover, simultaneous hydrogen and ethanol production were achieved by coupling E. harbinese B49 and R. faecalis RLD-53 in the IDPFR. According to stoichiometry, the hydrogen and ethanol production efficiencies were 82.67% and 82.19%, respectively. Therefore, IDPFR was an effective strategy for coupling DFB and PFB to fulfill efficient energy recovery from waste biomass.

【 授权许可】

   
2015 Liu et al.; licensee BioMed Central.

【 图 表 】

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