Electrophoretic characterization of species of fibronectin bearing sequences from the N-terminal heparin-binding domain in synovial fluid samples from patients with osteoarthritis and rheumatoid arthritis

Arthritis Research & Therapy

John H Peters²  Steven Carsons⁴  Mika Yoshida¹  Fred Ko¹  Skye McDougall³  Grace A Loredo²  Theodore J Hahn³ 

[1] Geriatric Research, Education and Clinical Center, West Los Angeles VA Medical Center, VA Greater Los Angeles Healthcare System, Los Angeles, CA, USA

[2] Sacramento VA Medical Center, VA Northern California Health Care System, Mather, CA, USA

[3] University of California, Los Angeles School of Medicine, Los Angeles, CA, USA

[4] Winthrop University Hospital, Mineola, NY, USA

关键词: synovial fluid;    rheumatoid arthritis;    osteoarthritis;    fibronectin;    chondrocytes;   

Others  :  1101338
DOI  :  10.1186/ar1001

 received in 2003-01-02, accepted in 2003-08-15,  发布年份 2003

【 摘 要 】

Fragments of fibronectin (FN) corresponding to the N-terminal heparin-binding domain have been observed to promote catabolic chondrocytic gene expression and chondrolysis. We therefore characterized FN species that include sequences from this domain in samples of arthritic synovial fluid using one-and two-dimensional (1D and 2D) Western blot analysis. We detected similar assortments of species, ranging from ~47 to greater than 200 kDa, in samples obtained from patients with osteoarthritis (n = 9) versus rheumatoid arthritis (n = 10). One of the predominant forms, with an apparent molecular weight of ~170 kDa, typically resolved in 2D electrophoresis into a cluster of subspecies. These exhibited reduced binding to gelatin in comparison with a more prevalent species of ~200+ kDa and were also recognized by a monoclonal antibody to the central cell-binding domain (CBD). When considered together with our previous analyses of synovial fluid FN species containing the alternatively spliced EIIIA segment, these observations indicate that the ~170-kDa species includes sequences from four FN domains that have previously, in isolation, been observed to promote catabolic responses by chondrocytes in vitro: the N-terminal heparin-binding domain, the gelatin-binding domain, the central CBD, and the EIIIA segment. The ~170-kDa N-terminal species of FN may therefore be both a participant in joint destructive processes and a biomarker with which to gauge activity of the arthritic process.

【 授权许可】

   
2003 Peters et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

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