Total solids content: a key parameter of metabolic pathways in dry anaerobic digestion

Biotechnology for Biofuels

Jean-Charles Motte¹  Eric Trably¹  Renaud Escudié¹  Jérôme Hamelin¹  Jean-Philippe Steyer¹  Nicolas Bernet¹  Jean-Philippe Delgenes¹  Claire Dumas¹ 

[1] INRA, UR0050, Laboratoire de Biotechnologie de l′Environnement, Avenue des Etangs, Narbonne F-11100, France

关键词: Lignocellulosic residues;    Moisture content;    Fermentative metabolites;    Dark fermentation;    Biohydrogen;   

Others  :  794633
DOI  :  10.1186/1754-6834-6-164

 received in 2013-06-18, accepted in 2013-11-13,  发布年份 2013

【 摘 要 】

Background

In solid-state anaerobic digestion (AD) bioprocesses, hydrolytic and acidogenic microbial metabolisms have not yet been clarified. Since these stages are particularly important for the establishment of the biological reaction, better knowledge could optimize the process performances by process parameters adjustment.

Results

This study demonstrated the effect of total solids (TS) content on microbial fermentation of wheat straw with six different TS contents ranging from wet to dry conditions (10 to 33% TS). Three groups of metabolic behaviors were distinguished based on wheat straw conversion rates with 2,200, 1,600, and 1,400 mmol.kg_VS^-1 of fermentative products under wet (10 and 14% TS), dry (19 to 28% TS), and highly dry (28 to 33% TS) conditions, respectively. Furthermore, both wet and dry fermentations showed acetic and butyric acid metabolisms, whereas a mainly butyric acid metabolism occurred in highly dry fermentation.

Conclusion

Substrate conversion was reduced with no changes of the metabolic pathways until a clear limit at 28% TS content, which corresponded to the threshold value of free water content of wheat straw. This study suggested that metabolic pathways present a limit of TS content for high-solid AD.

【 授权许可】

   
2013 Motte et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 3.

Figure 3.

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