Eubacteria and archaea communities in seven mesophile anaerobic digester plants in Germany

Biotechnology for Biofuels

Christian Abendroth³  Cristina Vilanova⁴  Thomas Günther⁵  Olaf Luschnig²  Manuel Porcar¹ 

[1] Fundació General de la Universitat de València, València, Spain

[2] BioEnergie Verbund e.V., Im Steinfeld 10, Jena, 07751, Germany

[3] Bio H2 Energy GmbH, Im Steinfeld 10, Jena, 07751, Germany

[4] Cavanilles Institute of Biodiversity and Evolutionary Biology, Universitat de València, Valencia, 46020, Spain

[5] Eurofins Umwelt Ost GmbH, Löbstedter Straße 78, Jena, 07749, Germany

关键词: Anaerobic digesters;    Methanogens;    Archaea;    Eubacteria;    Biogas;   

Others  :  1219215
DOI  :  10.1186/s13068-015-0271-6

 received in 2015-01-23, accepted in 2015-06-09,  发布年份 2015

【 摘 要 】

Background

Only a fraction of the microbial species used for anaerobic digestion in biogas production plants are methanogenic archaea. We have analyzed the taxonomic profiles of eubacteria and archaea, a set of chemical key parameters, and biogas production in samples from nine production plants in seven facilities in Thuringia, Germany, including co-digesters, leach-bed, and sewage sludge treatment plants. Reactors were sampled twice, at a 1-week interval, and three biological replicates were taken in each case.

Results

A complex taxonomic composition was found for both eubacteria and archaea, both of which strongly correlated with digester type. Plant-degrading Firmicutes as well as Bacteroidetes dominated eubacteria profiles in high biogas-producing co-digesters; whereas Bacteroidetes and Spirochaetes were the major phyla in leach-bed and sewage sludge digesters. Methanoculleus was the dominant archaea genus in co-digesters, whereas Methanosarcina and Methanosaeta were the most abundant methanogens in leachate from leach-bed and sewage sludge digesters, respectively.

Conclusions

This is one of the most comprehensive characterizations of the microbial communities of biogas-producing facilities. Bacterial profiles exhibited very low variation within replicates, including those of semi-solid samples; and, in general, low variation in time. However, facility type correlated closely with the bacterial profile: each of the three reactor types exhibited a characteristic eubacteria and archaea profile. Digesters operated with solid feedstock, and high biogas production correlated with abundance of plant degraders (Firmicutes) and biofilm-forming methanogens (Methanoculleus spp.). By contrast, low biogas-producing sewage sludge treatment digesters correlated with high titers of volatile fatty acid-adapted Methanosaeta spp.

【 授权许可】

   
2015 Abendroth et al.

【 图 表 】

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