Metabolic fingerprinting of Angelica sinensis during growth using UPLC-TOFMS and chemometrics data analysis

Chemistry Central Journal

Yujing Sun²  Yingwen Yang²  Xinbo Pan²  Hui Yan¹  Rina Sa²  Yali Wang²  Yiyun Qian² 

[1]Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing, China

[2]Key Laboratory of Dunhuang Medicine and Transformation, Ministry of Education, Research Institute of Angelica sinensis, Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China

关键词: Chemometrics;    Principal component analysis (PCA);    Metabolomics;    UPLC-QTOFMS;    Angelica sinensis;   

Others  :  787950
DOI  :  10.1186/1752-153X-7-42

 received in 2013-01-09, accepted in 2013-02-21,  发布年份 2013

【 摘 要 】

Background

The radix of Angelica sinensis is widely used as a medicinal herbal and metabolomics research of this plant during growth is necessary.

Results

Principal component analysis of the UPLC-QTOFMS data showed that these 27 samples could be separated into 4 different groups. The chemical markers accounting for these separations were identified from the PCA loadings plot. These markers were further verified by accurate mass tandem mass and retention times of available reference standards. The study has shown that accumulation of secondary metabolites of Angelica sinensis is closely related to the growth periods.

Conclusions

The UPLC-QTOFMS based metabolomics approach has great potential for analysis of the alterations of secondary metabolites of Angelica sinensis during growth.

【 授权许可】

   
2013 Qian et al; licensee Chemistry Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

Figure 6.

Figure 7.

Figure 8.

【 参考文献 】

• [1]China Pharmacopoeia Committee: Pharmacopoeia of the People’s Republic of China. Beijing, China: China Chemical Industry Press; 2010:124.
• [2]Wagner H, Bauer R, Xiao PG: Chinese Drug Monographs and Analysis. Angelica sinensis 2001, 3:1-2.
• [3]Yi LZ, Liang YZ, Wu H, Yuan DL: The analysis of Radix Angelicae Sinensis (Danggui). J Chromatogr A 2009, 1216:1991-2001.
• [4]Fiehn O: Metabolomics - the link between genotypes and phenotypes. Plant Mol Biol 2002, 48:155-171.
• [5]Verouden MPH, Westerhuis JA, van der Werf MJ, Smilde AK: Exploring the analysis of structured metabolomics data. Chemometrics and Intelligent Laboratory Systems 2009, 98:88-96.
• [6]Castillo S, Gopalacharyulu P, Yetukuri L, Orešič M: Algorithms and tools for the preprocessing of LC–MS metabolomics data. Chemometrics and Intelligent Laboratory Systems 2011, 108:23-32.
• [7]Ioset KN, Nyberg NT, Van Diermen D, Malnoe P, Hostettmann K, Shikov AN, Jaroszewski JW: Metabolic profiling of Rhodiola rosea rhizomes by 1H NMR spectroscopy. Phytochem Anal 2011, 22:158-165.
• [8]Gad HA, El-Ahmady SH, Abou-Shoer MI, Al-Azizi MM: Application of Chemometrics in Authentication of Herbal Medicines: A Review. Phytochem Anal 2012, n/a:n/a.
• [9]Xue SY, Li ZY, Zhi HJ, Sun HF, Zhang LZ, Guo XQ, Qin XM: Metabolic fingerprinting investigation of Tussilago farfara L. by GC–MS and multivariate data analysis. Biochem Syst Ecol 2012, 41:6-12.
• [10]Zhi HJ, Qin XM, Sun HF, Zhang LZ, Guo XQ, Li ZY: Metabolic Fingerprinting of Tussilago farfara L. Using 1H-NMR Spectroscopy and Multivariate Data Analysis. Phytochem Anal 2012, 23:492-501.
• [11]Jansen JJ, Smit S, Hoefsloot HCJ, Smilde AK: The photographer and the greenhouse: how to analyse plant metabolomics data. Phytochem Anal 2010, 21:48-60.
• [12]Tianniam S, Tarachiwin L, Bamba T, Kobayashi A, Fukusaki E: Metabolic profiling of Angelica acutiloba roots utilizing gas chromatography–time-of-flight–mass spectrometry for quality assessment based on cultivation area and cultivar via multivariate pattern recognition. J Biosci Bioeng 2008, 105:655-659.
• [13]Xiang Z, Wang XQ, Cai XJ, Zeng S: Metabolomics Study on Quality Control and Discrimination of Three Curcuma Species based on Gas Chromatograph-Mass Spectrometry. Phytochem Anal 2011, 22:411-418.
• [14]Ma C, Wang H, Lu X, Xu G, Liu B: Metabolic fingerprinting investigation of Artemisia annua L. in different stages of development by gas chromatography and gas chromatography–mass spectrometry. J Chromatogr A 2008, 1186:412-419.
• [15]Yi LZ, Yuan DL, Liang YZ, Xie PS, Zhao Y: Fingerprinting alterations of secondary metabolites of tangerine peels during growth by HPLC–DAD and chemometric methods. Anal Chim Acta 2009, 649:43-51.
• [16]Allwood JW, Goodacre R: An introduction to liquid chromatographyâ mass spectrometry instrumentation applied in plant metabolomic analyses. Phytochem Anal 2010, 21:33-47.
• [17]De Vos RCH, Moco S, Lommen A, Keurentjes JJB, Bino RJ, Hall RD: Untargeted large-scale plant metabolomics using liquid chromatography coupled to mass spectrometry. Nat Protoc 2007, 2:778-791.
• [18]Chan ECY, Yap SL, Lau AJ, Leow PC, Toh DF, Koh HL: Ultra-performance liquid chromatography/time-of-flight mass spectrometry based metabolomics of raw and steamed Panax notoginseng. Rapid Commun Mass Spectrom 2007, 21:519-528.
• [19]Dan M, Su M, Gao X, Zhao T, Zhao A, Xie G, Qiu Y, Zhou M, Liu Z, Jia W: Metabolite profiling of Panax notoginseng using UPLC–ESI-MS. Phytochemistry 2008, 69:2237-2244.
• [20]Li SL, Song JZ, Qiao CF, Zhou Y, Qian K, Lee KH, Xu HX: A novel strategy to rapidly explore potential chemical markers for the discrimination between raw and processed Radix Rehmanniae by UHPLC-TOFMS with multivariate statistical analysis. J Pharm Biomed Anal 2010, 51:812-823.
• [21]Lin LZ, He XG, Lian LZ, Wayne K, Jerry E: Liquid chromatographic–electrospray mass spectrometric study of the phthalides of Angelica sinensis and chemical changes of Z-ligustilide. J Chromatogr A 1998, 810:71-79.
• [22]Wang YL, Liang YZ, Chen BM: High-performance liquid chromatography with atmospheric pressure chemical ionization and electrospray ionization mass spectrometry for analysis of Angelica sinensis. Phytochem Anal 2007, 18:265-274.
• [23]Lu GH, Chan K, Liang YZ, Leung K, Chan CL, Jiang ZH, Zhao ZZ: Development of high-performance liquid chromatographic fingerprints for distinguishing Chinese Angelica from related umbelliferae herbs. J Chromatogr A 2005, 1073:383-392.
• [24]Liu HM, Liu XZ, Liu XR, Wang XZ: Effects of cultivation methods on dry matter accumulating and growth dynamics of Angelica sinensis. Chin Tradit Herbal Drugs 2007, 38:257-261.
• [25]Sun HM, Zhang BG: Investigation on the Growth Activities of Angelica sinensis(oliv.) Diels. in Gansu Region. Chin Agric Sci Bull 2010, 26:386-389.
• [26]Xu JZ, Qi FP, Jing YM, Liu MR, Zhao R, Li QP, Cai RQ: Studies on the Dynamic of Angelicae sinensis in Medicine Formation Period. Chin Med Mat 1997, 20:325-327.