The quantitative proteomic response of Synechocystis sp. PCC6803 to phosphate acclimation

Aquatic Biosystems

Phillip C Wright⁴  Kenneth F Reardon³  Catherine A Biggs⁴  Geoff McMullan²  Nigel G Ternan²  Josseilin Noirel⁴  Chee Sian Gan¹  Saw Yen Ow⁴  Matthew A Fuszard⁵ 

[1]Agilent Technologies, Singapore, Singapore

[2]School of Biomedical Sciences, University of Ulster, Coleraine, County Londonderry, BT52 1SA, UK

[3]Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO, 80523-1370, USA

[4]ChELSI Institute, Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, S1 3JD, UK

[5]BSRC Mass Spectrometry and Proteomics Facility, Department of Chemistry, University of St Andrews, St Andrews, KY16 9ST, UK

关键词: Synechocystis;    Ribose sugars;    Pentose phosphate pathway;    Phycobilisome;    Phosphate acclimation;    iTRAQ;    Cyanobacteria;   

Others  :  794233
DOI  :  10.1186/2046-9063-9-5

 received in 2012-06-08, accepted in 2013-01-28,  发布年份 2013

【 摘 要 】

Background

Inorganic phosphate (P_i) is a critical nutrient for all life and is periodically limiting in marine and freshwater provinces, yet little is understood how organisms acclimate to fluctuations in P_i within their environment. To investigate whole cell adaptation, we grew Synechocystis sp. PCC6803, a model freshwater cyanobacterium, in 3%, and 0.3% inorganic phosphate (P_i) media. The cells were allowed to acclimate over 60 days, and cells were harvested for quantitative high throughput mass spectrometry-based proteomics using the iTRAQ™ labelling technology.

Results

In total, 120 proteins were identified, and 52 proteins were considered differentially abundant compared to the control. Alkaline phosphatase (APase) activities correlated significantly (p < 0.05) with observed relative PhoA abundances. PstS1 and PstS2 were both observed, yet PstS1 was not differentially more abundant than the control. Phycobilisome protein abundances appeared to be coordinated, and are significantly less abundant in 0.3% P_i than 3% P_i cultures. Also, the central metabolic cell function appears to have shifted towards the production of (NADPH) reducing energy and nucleotide sugars.

Conclusions

This acclimation response bears strong similarity to the previously reported response to nitrogen deprivation within Synechocystis sp. PCC 6803. However, it also demonstrates some characteristics of desiccation stress, such as the regulation of fatty acids and increased abundance of rehydrin in the 3% P_i culture.

【 授权许可】

   
2013 Fuszard et al.; licensee BioMed Central Ltd.

【 图 表 】

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