Photodegradation assessment of ciprofloxacin, moxifloxacin, norfloxacin and ofloxacin in the presence of excipients from tablets by UPLC-MS/MS and DSC

Chemistry Central Journal

Urszula Hubicka¹  Paweł Żmudzki²  Przemysław Talik¹  Barbara Żuromska-Witek¹  Jan Krzek¹ 

[1] Department of Inorganic and Analytical Chemistry, Jagiellonian University Medical College, Faculty of Pharmacy, 9 Medyczna Street, 30-688 Kraków, Poland

[2] Department of Medicinal Chemistry, Jagiellonian University Medical College, Faculty of Pharmacy, 9 Medyczna Street, 30-688 Kraków, Poland

关键词: Differential scanning calorimetry;    Tandem mass spectrometry;    Ultra performance liquid chromatography;    Kinetic evaluation;    Photodegradation;    Ofloxacin;    Norfloxacin;    Moxifloxacin;    Ciprofloxacin;   

Others  :  787860
DOI  :  10.1186/1752-153X-7-133

 received in 2013-04-19, accepted in 2013-07-15,  发布年份 2013

【 摘 要 】

Background

Ciprofloxacin (CIP), moxifloxacin (MOX), norfloxacin (NOR) and ofloxacin (OFL), are the antibacterial synthetic drugs, belonging to the fluoroquinolones group. Fluoroquinolones are compounds susceptible to photodegradation process, which may lead to reduction of their antibacterial activity and to induce phototoxicity as a side effect. This paper describes a simple, sensitive UPLC-MS/MS method for the determination of CIP, MOX, NOR and OFL in the presence of photodegradation products.

Results

Chromatographic separations were carried out using the Acquity UPLC BEH C₁₈ column; (2.1 × 100 mm, 1.7 μm particle size). The column was maintained at 40°C, and the following gradient was used: 0 min, 95% of eluent A and 5% of eluent B; 10 min, 0% of eluent A and 100% of eluent B, at a flow rate of 0.3 mL min^-1. Eluent A: 0.1% (v/v) formic acid in water; eluent B: 0.1% (v/v) formic acid in acetonitrile. The method was validated and all the validation parameters were in the ranges acceptable by the guidelines for analytical method validation. The photodegradation of examined fluoroquinolones in solid phase in the presence of excipients followed kinetic of the first order reaction and depended upon the type of analyzed drugs and coexisting substances. Photodegradation process of analyzed drugs was confirmed by differential scanning calorimetry. In addition, the identification of degradation products was carried out by mass spectrometry.

Conclusion

The developed UPLC-MS/MS method enables the determination of CIP, MOX, NOR and OFL in the presence of photodegradation products and identification of photodegradation products.

【 授权许可】

   
2013 Hubicka et al.; licensee Chemistry Central Ltd.

【 图 表 】

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